[ccp4bb] Deadline EMBO Practical Course on X-ray crystal structure determination of macromolecules

2008-05-06 Thread Rob Meijers
Dear Community, this is a gentle reminder that the deadline for applications (15th of May) is approaching for the EMBO Practical Course X-ray crystal structure determination of macromolecules at the Synchrotron Soleil from 14 - 21 September 2008 This course is meant as an introduction

Re: [ccp4bb] negative LLG in Phaser run

2008-05-06 Thread junfeng liu
Dear Oganesyan, That means the second component is not right based on the number of LLG and PACK. Please see that for more details. http://www-structmed.cimr.cam.ac.uk/phaser/faq.html You should check that model and redo MR again. Good luck! leo Oganesyan, Vaheh wrote: Phaser experts,

[ccp4bb] PEPC6

2008-05-06 Thread Jochen Muller-Dieckmann
This is a reminder for the upcoming EMBO practical workshop on protein expression, purification and crystallization (PEPC6). The deadline for this workshop is May 25th. Applications are on-line only under http://www.embl-hamburg.de/pepcwww.embl-hamburg.de/pepc. The EMBL Hamburg

Re: [ccp4bb] Negative density around C of COO-

2008-05-06 Thread Nave, C (Colin)
Hi Just for completeness (seeing as we are discussing scattering factors and radiation damage) there is an increased susceptibility to primary damage for sulphur due to the increased absorption which follows f'', tabulated for example in http://henke.lbl.gov/optical_constants/asf.html. The x-ray

Re: [ccp4bb] protein-protein docking programs

2008-05-06 Thread Jerry McCully
Hi, All: Thanks a lot for the prompt reply from all of you. Probably I have another problem. My complex was mediated by two separated interfaces between the receptor and two subunits of the ligand. I tried HEX and the ClusPro server to dock the

Re: [ccp4bb] Refmac

2008-05-06 Thread Garib Murshudov
If you are using new version of refmac from www.ysbl.york.ac.uk/YSBLPrograms/index.jsp then you can use harmonic restraints. Have a look for instructions: www.ysbl.york.ac.uk/refmac/data/refmac_news.html I hope it helps. regards Garib On 6 May 2008, at 16:39, Debasish Chattopadhyay wrote:

[ccp4bb] posdoctoral position at UC Santa Cruz

2008-05-06 Thread Harry Noller
Postdoctoral position in X-ray crystallography of functional complexes of ribosomes available beginning in Fall 2008. This project is aimed at solving the structures of ribosomes trapped in intermediate states of the translational cycle, containing translation mRNA, tRNAs, translation factors,

[ccp4bb] posdoctoral position at UC Santa Cruz

2008-05-06 Thread Harry Noller
Postdoctoral position in X-ray crystallography of functional complexes of ribosomes available beginning in Fall 2008. This project is aimed at solving the structures of ribosomes trapped in intermediate states of the translational cycle, containing translation mRNA, tRNAs, translation factors,

Re: [ccp4bb] Negative density around C of COO-

2008-05-06 Thread James Holton
Indeed there are radicals, but they are not free. Perhaps a better terminology for them would be fixed radicals. Species such as the disulphide radical have been well established by spectrophotometric and other evidence (M. Weik et. al. and others). It is quite remarkable actually how

Re: [ccp4bb] COOT ramachandran plot

2008-05-06 Thread Paul Emsley
In message [EMAIL PROTECTED] Yanming Zhang [EMAIL PROTECTED] writes: Hi, all, If I have a large molecule, say 2000 aa, the coot ramachandran plot will be lots of outliers. Now I want to do things one step at a time, i.e checking the outliers by residue ranges such as, 1--500, 501--1000, so