Dear ccp4bb,
I have heard from many people that molprobity is the gold standard as
far as validation is concerned. As I am just about to deposit a
structure I therefore figured it was best to use molprobity in guiding
the final stages of my refinement. I do not have enough data to
Simon,
You should always include H atoms in refinement whatever the resolution,
I can't think of any reason for not doing so. They are not refined
independently but 'ride' on the parent atom, so don't contribute to the
parameter and restraint counts, and as you say it is essential that they
are
Dear All,
I would like to draw your attention to the following MX Beamline Scientist
positions currently open at Diamond to work on Beamlines I24 (Microfocus) and
I02.
All the best
Gwyndaf
===
Dear all,
I am sorry to bother you with two off-topic questions.
Question one:
I have solved a pig mitochondrial protein crystal structure whose molecular
weight is about 95kd. However, I do not have the sequence information of this
protein. It is weird that I could not blast its sequence in
Dear CCP4bb,
Sorry for the non-CCP4 related question.
I am running HKL2000 (ver 0.98.698m) on an iMac (OSX 10.5). On starting
up the software the following message is displayed:-
Total amount of memory found: 2041 MB (required by HKL-2000; 256 MB)
Could not check system resources
Please
only tried once). Does any body have ideas about how to extract mRNA from pig live (or animal tissue in general)?
From pig live? Yeah sure, toss it in a blender -- you can tell whether
it's alive by the squealing. You may need quite a large blender,
though, and a crane to lift it, pigs are
Do we eally have to idicule postes to the ccp4 boad because of a missing
lette? If evey post with a othogaphic mistake elicited such a eaction,
I doubt anyone would cae to post to the bulletin boad.
Andeas
Frank von Delft wrote:
only tried once). Does any body have ideas about how to
Oh, snap!
Frank von Delft [EMAIL PROTECTED]
Sent by: CCP4 bulletin board CCP4BB@JISCMAIL.AC.UK
05/12/2008 09:12 AM
Please respond to
Frank von Delft [EMAIL PROTECTED]
To
CCP4BB@JISCMAIL.AC.UK
cc
Subject
Re: [ccp4bb] Two off-topic questions
only tried once). Does any body have
Sorry that I missed a letter. I wanted to extract mRNA from pig liver. Thank
you, Frank, for having taught me a lesson. I will carefully check my emails
before I send them to ccp4bb.
I am looking forward to getting suggestions.
Best wishes
Wei Yong
I had the same problem with the zoom window, so I just downloaded the
older version of HKL2000 and replaced the old version of xdisp (the zoom
window app.) with the new version. I'm not sure why, but it now works
perfectly.
Hope that helps,
Eric Salgado
Balvinder Dhaliwal wrote:
Dear
Sorry, but I realized my reply was confusing:
I replaced the new version of xdisp on the new working HKL2000 with the
old application, that way the only thing not updated is xdisp.
Eric Salgado
I had the same problem with the zoom window, so I just downloaded the
older version of HKL2000 and
Or, make sure that you don't post it on Monday, some people, sometimes
are in very bad mood on Mondays, I am not going to explain why.. :P
On Mon, May 12, 2008 at 5:57 PM, Yong, Wei [EMAIL PROTECTED] wrote:
Sorry that I missed a letter. I wanted to extract mRNA from pig liver. Thank
you,
Hello there, I don't usually do this, but the missing letter reminded me
of thiseverything is relative!
More Brain Stuff . . . From Cambridge University
O lny srmat poelpe can raed tihs.
cdnuolt blveiee taht I cluod aulaclty uesdnatnrd waht I was rdanieg.
The
phaonmneal pweor of
Hi,
Did you install SHARP/AutoSHARP in macbook with intel core 2 duo (OSX
10.5)? I need to install it. I fulfilled all the requirements except 'apache
httpd'. Would you like to tell me in detail exactly which one(apache httpd
2.2.8) I should download and how to install it. Thanks for your
Hi,
you don't need to install your own httpd: we do ship a httpd binary
ready for use on your machine.
By far the easiest is to follow the instructions on
http://www.globalphasing.com/sharp/installation/scenarios.html#oneone
which boil down to
1. get a few files from us
2. run
%
hloy cow! taht msut be vrey crortcet idened !
rjai
-Included Message--
Date: 12-may-2008 14:15:03 -0400
From: Scapin, Giovanna [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Two off-topic questions
Hello there, I don't usually do this, but the missing letter
Greetings CCP4 posters:
Below is a job posting for: Associate Director, Protein Structure
Laboratory at the University of Kansas
Position Description: The University of Kansas has an immediate career
opportunity for a highly motivated and experienced scientist to lead the
Protein Structure
Are they Lancashire Pigs?
On 13/05/2008, at 12:12 AM, Yong, Wei wrote:
Dear all,
I am sorry to bother you with two off-topic questions.
Question one:
I have solved a pig mitochondrial protein crystal structure whose
molecular weight is about 95kd. However, I do not have the sequence
Dear All,
The Berkeley Center for Structural Biology (BCSB) has recently completed
a major upgrade of the Sector 5.0 beamline optics. A five- to tenfold
improvement in available flux has been achieved at the side-station beamlines
(5.0.1 and 5.0.3), which now generate 1.5x10^11 photons per
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