Dear CCP4 Community,
My apologies for the off topic question to the bb.
I am trying to crystallise a small protein-protein complex. I purify as a
complex protein after expression in BL21DE3 cells through NI-NTA affinity
chromatography. pI of one of the protein is around 10.6 where as another
What protein concentration are you using in your screen? It sounds like
you need to try lower protein concentration, or a screen with lower
precipitant concentration, or both. Hampton makes a Crystal Screen Lite
with the precipitant concentrations half of what they are in the normal
screen (or
Dear Ron --
On 20 Sep 2008, at 15:59, Ron hudson wrote:
it is happily soluble and donot prcipitate at this pH in the same
buffer.
Just a quick question. How does your complex look like under DLS? You
might still have soluble aggregates, which will precipitate... you
can screen for
A postdoctoral position is immediately available in the structural biology lab
of McArdle Cancer Institute of University of Wisconsin at Madison to study
structures of signaling complexes related to cancer
(http://mcardle.oncology.wisc.edu/faculty/bio/xing.html). Strong background in
Dear friends and crystallographers,
During One of my lab meeting ,
I told twinning in crystals are ok, because ccp4's recent releases just need
the keyword TWIN to solve them,
As a new generation research student, I am now confused, is that I need to
learn and understand all programs(so
On Sep 20, 2008, at 2:18 PM, Jayashankar wrote:
Dear friends and crystallographers,
Are they mutually exclusive?
During One of my lab meeting ,
I told twinning in crystals are ok, because ccp4's recent releases
just need
the keyword TWIN to solve them,
I believe the closer you get
I agree with Bill.
After a few minutes thinking, in between jobs working in the yard:
It depends if you need to understand everything (I guess that's impossible
these days) --- are you comfortable with publishing and defending research
results that you do not understand? I am not. In quite a
On 06:56 Thu 18 Sep , William G. Scott wrote:
CCP4 6.0.99 builds its own libxml2. It there a way to get it to use a
later version I have already installed? The reason is that this is
messing up other programs that for some reason I can't fathom (the evil
$DYLD_LIBRARY_PATH remains
Hi,
In addition to the excellend comments posted already, I would like to
venture an opinion regarding learning, technology, and associated matters.
Do not make the problem fit the techniques you know - instead try to make
sure that you know (or know where to learn) enough techniques to
6.cryo crystallography
This is routine.
Well maybe there are still some surprises. Consider the case of high-
pressure cooling.
8.radiation damage
see cryocrystallography, and take lots of vitamin C
There may be some surprises here too as one looks at smaller crystals
with
Now that it is past 12:00 in Brookhaven, I want to be the first to
wish Bob Sweet a happy birthday!
Bob's brilliance is matched by his helpfulness, kindness and
compassion; he very much lives up to his name.
So on behalf of probably hundreds of us, happy birthday.
It's been awhile (too
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