Re: [ccp4bb] NCS and manual building

Dear Olga, I have also encountered the same problem. Find below a script which will automate the superposition of one protomer onto the others. Best wishes Prakash Superposition script :- #!/usr/bin/tcsh -f grep -v -E '^END' Aorig.pdb new.pdb rm mat1.mat foreach chain_name ( B C D E F

Re: [ccp4bb] RAxis fiber diffraction

Hello, You could try HELIX or FibreFix software, put crystalline DNA fibres into a capillary, no cryostream, shoot at full power for about an hour... from then it depends on the material. Regards, Mark _ Dr Mark Agacan Scientific Officer, Division of

Re: [ccp4bb] RAxis fiber diffraction

Sue, Louise Serpell at the University of Sussex, UK specialises on fibre diffraction and has some neat software that may be useful to your cause. You can contact her at [EMAIL PROTECTED] Klaus - Klaus

Re: [ccp4bb] Reducing Agents and pH

TCEP is often sold as hydrochloride salt and therefore reacts highly acidic. We usually titrate the concentrated solution *before* we add it to our proteins. This can be done within small volumes by dropwise addition of a saturated TRIS solution, check pH by pipetting a drop on a pH test

Re: [ccp4bb] RAxis fiber diffraction

Hi Sue, You said: Can anyone recommend some reading material or tutorials for me to learn more about what I've agreed to do? A nice tutorial on fiber diffraction is on Ken Holmes's website : http://www.mpimf-heidelberg.mpg.de/~holmes/ It might even work with other detectors ;-) ! Best wishes,

[ccp4bb] Monitor

I am looking for a good CRT monitor for crystallography that supports stereo well. CRT monitors particularly large 20 inch viewable are harder to find these days. Any suggestions on the best monitor? Are any large LCDs capable of stereo support? Brian

[ccp4bb] Monitor

I am looking for a good CRT monitor for crystallography that supports stereo well. CRT monitors particularly large 20 inch viewable are harder to find these days. Any suggestions on the best monitor? Are any large LCDs capable of stereo support? Brian

Re: [ccp4bb] twin axis in p21

Dear Hua, Your problem sounds like a 'false origin' one, where due to the pseudotranslation the molecular replacement choses the wrong origin out of the two possible ones. Your space group is probably P212121. The best approach is to run Andrey Lebedev's program ZANUDA on YSBL server.

Re: [ccp4bb] Resolve to CCP4...

Summary: A couple of people pointed out that refmac allows you to pick which Free set you use. Unfortunately, not all programs have this option, not all expose it via the GUI, and if you forget just once to change this from the default, you've messed up your free set. However, Tom