Hi Todd,
In case no one answered: We have several of those MVE dry shippers and
sometimes use them to store Rigaku pucks. The racks do not fit in nicely. You
have to use some force to get them into the shipper, but it works.
Cheers,
Dirk
---
Dr. Dirk Reinert
Boehringer Ingelheim Pharma GmbH
Hi all,
can anyone recommend an N-terminal sequencing facility in Germany, or nearby in
Europe, which does seqquencing of PVDF blotted protein?
Perhaps replies off-board are best and I can post a summary anyone interested.
Thanks in advance,
Tim Keys
PhD Student
Medizinische Hochschule
One thing I found with 2,3-Butanediol was for some crystallizations you can
make it up a cryo solution with it at 12% and then put a thin layer it on top
of the crystal droplet in a sitting drop well. Then you can loop the crystal
and pull it up through the cryo layer - then out and to N2(l).
Thanks to everyone who answered. There are many ways to display the images.
Summarized here are the answers:
- adxv
http://www.scripps.edu/~arvai/adxv.htmlhttp://www.scripps.edu/%7Earvai/adxv.html
Run it with the option -nopixmap if you are using it inside an NX
client
-
- There
What XPREP (and some other programs) calls Rint is the same as Rmerge
and is given by 100Sum|I-I|/Sum(I) where the sums are calculated
over all reflections with more than one equivalent (symmetry
equivalents and identical indices), I is the intensity of an
individual reflection and I is the mean
Dear CPP4 community,
I am in search of a program that will calculate the net charge of an
electrostatic surface formed by individual secondary structural elements
(versus the entire protein) of a structure: for example, the net charge
of the electrostatic surface of helices 2 and 3 versus,
Hi Pavel,
you should add to the explanation what /==1 and !=1 are, as the majority
of people don't know.
/
== : equal
!= : not equal
Maia
/
/
Pavel Afonine wrote:
Hi Regina,
this subject was discussed on PHENIX bulletin board some time ago:
Hello Rebecca,
this is merely a guess, but I think, it should work.
As you calculate the electrostatic surface potential in pymol, it uses apbs.
apbs, if I remember correctly creates an intermediate PDB-file where the
B-factor column is replaced with the partial charge of that atom.
So if you
Hi everybody,
can anybody tell me how to increase the number of lines in a mesh, when I
generated a electron density map in pymol? My mesh has to much space between
the lines.
The command min mesh spacing doesn't work, though I'm not sure, if this
the right command at all.
Thanks,
P.
One option is to use the map_double command to
make a finer mesh, e.g.,
map_double mymap.map, -1
The maps produced by CCP4 by default are typically too coarse.
Cheers.
On 3/25/2010 12:00 PM, Paul Lindblom wrote:
Hi everybody,
can anybody tell me how to increase the number of lines in a
A postdoc position in membrane protein structural biology is available in the
Biomolecular Research Laboratory (BMR) at the Paul Scherrer Institute,
Switzerland. The successful candidate will work on the production,
characterization, crystallization and structure determination of the serotonin
Uups: We're using MVE SC 4/2V. Dirk
Original-Nachricht
Datum: Thu, 25 Mar 2010 08:33:40 +0100
Von: Dirk Reinert dirk.rein...@boehringer-ingelheim.com
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] AW: [ccp4bb] \'Mushroom\' shipping dewar for Rigaku pucks
Hi Todd,
In case
Le 25 mars 2010 à 16:43, Tim Gruene a écrit :
Hello Rebecca,
this is merely a guess, but I think, it should work.
As you calculate the electrostatic surface potential in pymol, it uses apbs.
apbs, if I remember correctly creates an intermediate PDB-file where the
B-factor column is
My literature search is not going so well.
can anyone think of examples of the same substrate (not
oxidized/reduced, intermediates or analogs) bound to the same enzyme
(not mutants) binding site, but with one moiety adopting a drastically
different conformation between different
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