Rongjin,
It does look like you have the same problem I did. I was never totally able
to figure it out completely. First of all, it was only a problem with some
PDB files and not others. Modifying generic_objects.py (i.e., change the
line "if (reduce_status):" to "if (True):" ) as suggested by P
Hi,
I am assuming the enzyme is not active, or by substrate you do not mean the
actual substrate, may be an analogue. The substrate might be converted into
product and the leave through the channel and you will not find anything
bound to it. But I think you have taken care of that.
On Fri, Apr 9
One might also check the pH of your crystal and the number of hydrogen bond
donors from the putative sulfate (zero?) or the putative phosphate
(non-zero).
I'm alluding to the structures of the sulfate-binding protein and the
phosphate-binding protein from Quiocho's group.
Jim
_
From: CC
Hello,
Thanks for this interesting poll!
I have had similar doubt couple of times in the recent past.
I think in the absence of anomalous signal for confirming presence of
phosphate or sulphate, I would prefer to assign the electron density to the
one which is present in higher concentration in th
Dear colleagues,
I would like to poll the community on the prevailing practice of
distinguishing between phosphate and sulfate in their structures.
Suppose all of the following apply:
1) a well contoured tetrahedral density in your model-phased 2Fo-Fc map in
the active site of your kinase or GTPase
Chris
Isopropanol is said a very good precipitant but it's unpopular because it's
so difficult to harvest crystals
However there's a very convenient way to grow and harvest crystals in
isopropanol using Vapor Batch plates.
Basically you set up microbatch-under-oil drops containing all the
ingred
