[ccp4bb] Beam time available at X6A

2011-02-21 Thread Stojanoff, Vivian
Beam time available @ X6A http://protein.nsls.bnl.gov The NIGMS beam line X6A at the National Synchrotron Light Source provides FAST access to beam time through out the year. To apply submit a short proposal to http://protein.nsls.bnl.gov at any time. Proposals are continuously reviewed and

[ccp4bb] Deadline March 1, 2011: Travel Fellowships for XXII IUCr Congress, Madrid, August 22-31, 2011

2011-02-21 Thread Gloria Borgstahl
Please visit the USNC/Cr website for more information. *http://sites.nationalacademies.org/pga/biso/IUCr/*

Re: [ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

2011-02-21 Thread James Stroud
The pH is too low and the EDTA is insoluble. You need to be above pH ~8.0. Your best bet is to try another chelator. James On Feb 21, 2011, at 2:22 PM, Yibin Lin wrote: > Dear all, > > I got a lot of salt crystals in reservior solution (well solution), which > contains 0.1 M phosphate/citrate

[ccp4bb] Could someone can help me to explain why EDTA-2Na can formate salt crystals

2011-02-21 Thread Yibin Lin
Dear all, I got a lot of salt crystals in reservior solution (well solution), which contains 0.1 M phosphate/citrate ph 4.2, PEG200 47%, EDTA-2Na 0-22mM. Reservior solution appears crystals from 12mM EDTA. Could someone help me to explain why? Thank you very much! Yibin

Re: [ccp4bb] off-topic: Discovery Studio software

2011-02-21 Thread Francisco Hernandez-Guzman
Correct, the DS Visualizer is not available for Mac OS at this point, however various emulators such as Parallels or VMWare have been used successfully. Without going into too much detail, there is a new version (3.0) of the visualizer which extends even further the capabilities of our previous

Re: [ccp4bb] optimization of dumbbell like crystal

2011-02-21 Thread Anja Pomowski
Dear Harvey, I had very good results with low melt agarose and the detergent screen (e.g. Hampton Research). In both cases, the tendency for cluster formation as well as the diffraction power could be improved. Anja > Dear CCP4BBers, > > Recently I got a crystal which appeared in the condition

Re: [ccp4bb] optimization of dumbbell like crystal

2011-02-21 Thread Prince, D Bryan
Dear Harvey, Microseeding and adding 1-5% glycerol to the drops helped me when I obtained crystals like these. Good Luck! Bryan From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Harvey Rodriguez Sent: Sunday, February 20, 2011 9:29 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb

Re: [ccp4bb] off-topic: 2 peaks on Cation

2011-02-21 Thread Nadir T. Mrabet
In your original mail (2011-02-18; 18:45 GMT), you wrote: "Possibly deamidation of the protein, in particluar one or more lysines" Hence, my comment was based on your own writing, that is deamiDation. Wishing you a better week. Nadir Pr. Nadir T. Mrabet Structural& Molecular Biochemistry Nutri

[ccp4bb] Postdoc position at the Gene Center Munich

2011-02-21 Thread Dirk Kostrewa
Dear CCP4ers, on behalf of Patrick Cramer, I send a Postdoc position offer, given below. Please, do not respond to me, but to the e-mail addresses given in the job description. Best regards, Dirk Kostrewa. *** Applications are invited for

Re: [ccp4bb] optimization of dumbbell like crystal

2011-02-21 Thread yybbll
Additives screening. YB 2011-02-21 yybbll 发件人： Bosch, Juergen 发送时间： 2011-02-21 03:46:12 收件人： CCP4BB 抄送： 主题： Re: [ccp4bb] optimization of dumbbell like crystal Seeding ? changing from sitting to hanging drop or under oil ? play with temperature ? Jürgen On Feb 20, 2011, at 9:2