Hi,
Thank you very much.
In the example5 of this page
http://www.ysbl.york.ac.uk/~garib/refmac/docs/usage/examples.html#exam5, It
seems that for 3A dataset, MAKE
HYDRogenshttp://www.ysbl.york.ac.uk/%7Egarib/refmac/docs/keywords/restraints.html#make_hydrNo.
Is it mean that the hydrogen just
Hello and good day to everyone! :)
I have some general questions on crystallography work. I hope you don't mind
giving me some ideas.
I have solved my lipase protein both ground-grown crystals and space-grown
crystals with good resolutions (1.4A and 2.2A). They are the same protein
from the same
When in doubt, try both. In my personal experience, adding hydrogens always
works. Especially at low resolution. But don't take my word for it, experiment
a little.
Cheers,
Robbie
Date: Sun, 10 Jul 2011 16:01:59 +0800
From: caiq...@gmail.com
Subject: Re: [ccp4bb] low resolution refinement
To:
Hi Fairolniza,
There is little detail to go on here - I'd double check everything including
the processing. All things being equal, even with reduced acceleration, you
should have the same crystal form.
That said, if there is a difference, I suspect it has little to do with any
reduced
Please clarify:
Did you process the sets separately from the images?
Or did you process 360 degrees of images all the way to scaled, but
unaveraged results, then average reflections from the 5 different rotation
ranges?
Or something else?
With most processing programs that I am aware of, one
OK, same space group, but you didn't indicate what the unit cells were.
They are different, right?
_
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of ferrol
shariff
Sent: Sunday, July 10, 2011 3:10 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Same protein,
I would not rule out pure chance. Crystals of the same protein can and often
do grow in two (or more!) different forms, from 'the same conditions' and
often in the very same drop. In this case it's the same space group, but
presumably different cell dimensions? Until a significant number of
Thank you very much everyone for the replies.
Ok. Here are more details on the work:
1) Crystallization was done using counter diffusion method- in a capillary
(for both Space and Ground experiments)
2) Vapor diffusion method was done only for Ground experiments, and produced
both Shape 1 and
Thank you for the clarification.
So you have two independent crystal forms from the 'same' condition and one
of them does not grow in space. Sounds like there is some basis to the
theory that lack of gravity caused the first form to disappear.
Unfortunately it can also be the subtle changes in
