Wow, it is quite a lecture here! It is very appreciated.
I admit some (most?) of my statements were questionable. Thus, I did not know
how sigI would be calculated in case of multiple observations, and, indeed, its
proper handling should make sigI/I similar to Rmerge. Consequently, I/sigI
At 280nm absorbs Trp but Tyr and Phe (much less) as well. So your protein
sample even without Trp will absorb. A mass spect experiment should show a
presence of molecule with MW 507 (ATP) or MW 427 (ADP)
George
Post-doctoral and PhD positions available in the group of Titia K. Sixma,
Netherlands Cancer Institute, Amsterdam
We are looking for motivated enthousiastic researchers with strong experience
in X-ray crystallography and protein biochemistry. Projects are focussing on
ubiquitin conjugation
well, actually i recommend having a look at the old but good scalepack manual
for why Rmerge is inferior..
(i thought this was clear long ago.. so i am bit amazed that this discussio is
still alive and kicking..)
question of where to cut, is a different one and thats where the recent papers
Ion exchange chromatography, this is the published method.
Acoot
From: Xinghua Qin xinghua...@126.com
To: CCP4BB@JISCMAIL.AC.UK
Sent: Monday, 4 June 2012 2:51 PM
Subject: [ccp4bb] How to know if ADP exists in the ATP-binding site of
bacterial expressed proteins
Deer CCP4ers,
How to know
Is it reasonable to refine occupancy in phenix at 2.2 A resolution?
Implementations may differ, but imgo refining occupancy at 2.2A
resolution is not very reasonable under most circumstances, as it will
correlate strongly with the B-factor. A reasonable approach might be to
fix occupancy at
On Sat, 2012-06-02 at 23:32 -0700, aaleshin wrote:
Was not Z. Otwinowski first to use it in his scalepack?
Maybe I missed something, but given the hoops I have to jump through to
get Rpim calculated after scalepack (basically take unmerged data to
either the program from Manfred Weiss or SCALA)
Dear colleagues,
This is a reminder that applications are still open for BIOCRYS 2012
Fundamentals of Modern Methods in Biocrystallography
organised by Maria Armenia Carrondo and Thomas R. Schneider
Course sponsored by : FEBS, BioStruct-X, IUCr, and IUBMB
*Date Location*
*20th - 27th
i thought this was clear long ago.. so i am bit amazed that this discussio is
still alive and kicking..
Even though it does not relate to me, but the explanation may come from the
fact that new people start using crystallography, and they do not like reading
old papers. So, there is nothing
Hi Ed,
I've actually run that exact test in phenix as an exercise to prove to my
PI the validity of occupancy refinement. Though as a disclaimer it was a
1.2 angstrom data set and this was an alternate conformation situation. I
ran different input occupancies without occupancy refinement and
On Mon, 2012-06-04 at 13:11 -0500, Katherine Sippel wrote:
Though as a disclaimer it was a 1.2 angstrom data set
Which is about 6x more data than 2.2A... Certainly, at atomic
resolution the results of occupancy refinement will be more robust. To
be fair, even at 2.2A such refinement may
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