Re: [ccp4bb] a challenge

2013-01-13 Thread Demetres D. Leonidas
Since the discussion for crystallographers is fired up. I want to put on record that I totally agree with Tassos about the profile of a crystallographer. If you take away the crystals, then a crystallographer is no long a crystallographer. Demetres On 13/1/2013 9:52 μμ, Anastassis Perrakis w

[ccp4bb] offtopic: steady state kinetics

2013-01-13 Thread Peter Hsu
Hi all, Sorry for the incredibly dumb and off topic question. I've been doing some steady state michaelis menten kinetics, and no one around me has ever done it before. My own classroom training was years ago and so I've forgotten a great deal and have just been going from old textbooks/papers.

Re: [ccp4bb] a challenge

2013-01-13 Thread jens Preben Morth
I agree with Tassos, and btw think that this crystallographer, should be able to go back into the lab and optimize the present crystal conditions to get better crystals. In particularly, when he or she realize that the scientific question they set out to investigate cannot be answered, by analy

Re: [ccp4bb] a challenge

2013-01-13 Thread Anastassis Perrakis
> I think the real challenge (and one that makes for an excellent > macromolecular crystallographer) is how well one can interpret a map with > poor phases. Let me disagree ... An excellent macromolecular crystallographer, is one that given some crystals can derive the best strategy to collect

Re: [ccp4bb] a challenge

2013-01-13 Thread Francis E Reyes
Ok, I'll bite. "I dare anyone who considers themself an expert macromolecular crystallographer to find a way to build out of this map." I put emphasis on "this map". "Short of actually cheating (see below), there doesn't seem to be any automated way to arrive at a solved structure from these

Re: [ccp4bb] a challenge

2013-01-13 Thread George Sheldrick
I have now looked at James's two challenges to see what I could learn from them, and will try to give enough details so that less experienced readers of this list can repeat what I did and apply the experience thereby gained to solving their own structures. For those who are not interested in