Dear Developers,
I wonder if there is any particular reason why, if present in an mmcif, cell
parameters and SG
are not read from the mmcif file when using 'convert to mtz'?
Best, BR
Hi Bernhard,
When I have used the convert program via ccp4i, the cell and
symmetry have indeed been read from the mmcif file and written to the
MTZ file.
Andrew
On 16 May 2013, at 13:50, Bernhard Rupp wrote:
Dear Developers,
I wonder if there is any particular reason why, if present
Yes, this is indeed what I see as well and it confused me too ...
those fields should not be orange highlighted according to my
understanding of the way CCP4i works.
Andrew
On 16 May 2013, at 14:33, Bernhard Rupp wrote:
Ah – let me rephrase that – cif2mtz ultimately seems to read them,
It looks like structured PEG to me. Did you have PEG as a precipitant in
you crystallization solution? This is a quite normal feature, you will find
many examples in the PDB.
You could try to model it and see how it fits the density, how your
R-factors behave, etc.
On Thu, May 16, 2013 at
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Research topic: Structure and function of large, multicomponent biological
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Dear All:
I thought that it is possible to be the PEG. The crystallization condition
contains 3% of PEG.
Thank you for your comments and input
Uma
On Thu, May 16, 2013 at 11:58 AM, Mario Sanches mario...@gmail.com wrote:
It looks like structured PEG to me. Did you have PEG as a precipitant
Just a few things to add:
If you have a _current_ version of ADXV, then that disappearing sharp
spots problem has been fixed. Try downloading a new copy. Its free.
To answer the OP's question: there was a paper written about practical
Pilatus data collection recently:
It is most likely to be something in your crystallisation condition,
your cryoprotectant, or the buffer you stored your protein in.
Could be a detergent carried through several steps of purification
Could for example be some part of a PEG molecule that gets ordered round
a more hydrophobic bit
Dear James,
A week ago I wrote what I thought was a perhaps excessively long and
overly dense message in reply to Theresa's initial query, then I thought I
should sleep on it before sending it, and got distracted by other things.
I guess you may well have used that whole week composing
Dear Gerard - thanks, very informative! Two questions:
1.
Do I understand correctly, that you say XDS will throw together for
integration counts from many images even if they're spaced widely
throughout the dataset, i.e. through the various passes?
i.e. if I set up my data collection as 5
I THINK IT IS PEG..
On Thu, May 16, 2013 at 10:20 PM, Nicholas Keep n.k...@mail.cryst.bbk.ac.uk
wrote:
It is most likely to be something in your crystallisation condition, your
cryoprotectant, or the buffer you stored your protein in.
Could be a detergent carried through several steps of
Dear Frank,
Very good question. You are right: in this statement I used XDS
rather metaphorically, in the sense that this grouping together of all the
counts will happen at merging time rather than at integration time. In the
end you will have a counting-statistics contribution to the
Gerard,
Thanks!
Actually, I was sitting on that one for a while and debating the wisdom
of posting it. With multi-million-dollar equipment, people tend to get
sensitive about opinions.
But, yes, I suppose I didn't answer Theresa's second question about
anomalous data collection and rad
Frank,
Kay or Wolfgang can obviously answer better, but I am pretty sure the
answer to your second question is no. XDS does not consider images
collected at 0 and 360 to be one image. In fact, I think the phi
direction is internally represented as detector Z, where Z has
units of the
Hi fork,
I am working with a membrane protein. I got some crystals from peg400.
All of these crystals don't diffract. I tried to use a needle to break
crystal. They didn't break into small one, look like gel, or oil,
something like that. Do anybody has deal about these crystals.
Detergent
And Kay Diedrichs suggested today that averaging out possible errors in the
Lorentz correction is a potential benefit of collecting real redundant data.
(this would apply to both SR and lab sources).
Colin
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From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
Hi all:
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