Re: [ccp4bb] shelx anamalous data

2013-11-16 Thread Eleanor Dodson
It is easy enough if you still ant to do it. I would feed both into f2mtz separately to make a mysadIplus.mtz and a mysadImin.mtz then use CAD to combine and change the default labels to something like I(+) SIGI(+) and I(-) SIGI(-) But as George says Why do you want it? That will change the

[ccp4bb] Fwd: Re: [ccp4bb] shelx anamalous data

2013-11-16 Thread George Sheldrick
*Dear Eleanor, There is a reason why I do not provide a routine to go from the .phs from shelxe to .mtz. If he uses the 'free lunch' option in shelxe he may finish up refining against the free lunch structure factors. According to one user who managed to do this by accident, this gives

[ccp4bb] Marie Curie Early Stage Research

2013-11-16 Thread Klaus Fütterer
Dear Colleagues, I would like to make you aware of the following opportunity for final year undergraduate and Masters-level students. The University of Birmingham and GSK have initiated Coopera-TB, an EU-funded consortium focussing on hit to lead optimisation of novel anti-TB scaffolds.

Re: [ccp4bb] Oligomerization of maltose-binding protein

2013-11-16 Thread Xiao Xiao
Hi Zhijie, Thank you so much for the reply. The protein I am working on is from eukaryotic cytosol. We've already tried optimize the IPTG concentration and induction temp. Co-expression of chaperons seems to be a interesting idea, I googled and found this one: