The best way to ascertain whether you have any meaningful crystal is
diffraction. However, in the past, I have collected data from crystals that did
not look "very sharp" or looked kind of oval in shape.
To manage or fine tune your crystallization trials you can either try
microbatch (under oil
Some of the best data I've collected came from ovoid, or outright spherical
crystals. Naturally, on other occasions very similar looking crystals
produced diffraction patterns similar to those of a Sony Walkman... which
just goes to show that crystal habit is an unreliable indicator of crystal
qual
I don't know why you would concern yourself with the outward morphology
of the crystal, rather than its diffraction quality. I have grown some
gorgeous little gems which did not diffract well.
On 12/24/13 23:49, Acoot Brett wrote:
Dear All,
I am optimizing a crystal. In one of the optimizing
The Paul Scherrer Institute (PSI) is the largest national research institute
in Switzerland and operates, among other large-scale facilities, the Swiss
Light Source (SLS), one of the most advanced synchrotron radiation sources
worldwide. The Macromolecular Crystallography (MX) group at the SLS
(htt
Well it could be PEG, I have seen these things with rough edges/surface
looks bit crystalline,but never diffracted.
But it could be different in your case, can't say without much information.
Check if your protein sample to be pure ( impurities also influence
crystal growth), or degradation of pr
Dear Acoot,
it is not unusual that crystals have an optimum period of growth, and
you may get away with storing the crystal once it appeared to collect
data from it.
Otherwise I recommend you think about what is happening inside the well
during this day in order to further optimise the crystal qu
