Dear Sudipta,
you are correct, your original scala.mtz has the wrong space group in it,
resulting in very high Rfactors (and presumably bad electron density).
In these cases, I usually reprocess (remerge) the data in the correct space
group to get the statistics right (and gain probably a few
Dear Sudipta—
Herman is correct, you just need to reindex your scala.mtz file to the correct
space group.
Regarding translational NCS, it has been many years since I’ve dealt with this,
but it is my impression that modern refinement methods treat this much better,
especially with maximum
Please can you remove me from the mailing list.
Thank you
Natalie Collett
Communications Officer
Instruct: Integrating Structural Biology, Wellcome Trust Centre for Human
Genetics, University of Oxford, Roosevelt Drive, Headington OX3 7BN, UK
Tel: +44 1865 287711
email:
On Mon, Jun 30, 2014 at 09:42:43PM -0500, Maher Alayyoubi wrote:
Hi everyone,
I have two questions:
1- I was trying to run the program Acorn, on a SAD dataset (Se derivative)
that was scaled in scalepack/HKL2000. converted to .mtz using
scalepack2mtz, then edited in REVISE and Ecalc. But
I have been attempting to obtain a protein crystal of my protein for just over
2 years at this point. We have attempted removing the tag, binding the protein
to its ligand, removing as much salt as possible (crashes at at too low a salt
concentration)--this lead us to try reverse vapor
Hi Catherine,
If they are indeed salt crystals, have you tried preparing different
truncations of the gene encoding your target? I've seen a number of
successes in which, after a codon or two was added to the termini of the
gene being overexpressed, the target crystallized beautifully.
Best,
Hi Catherine,
What buffer salt do you have your protein in when you set up screens -
maybe this is leading to your salt crystal issues? How long does it take
for the salt crystals to appear?
Have you tried setting up trays at different temperatures? Microbatch
crystallisation under oil?
Hi Catherine,
first of all, I'm sorry that you're feeling so frustrated.
In addition to the many sensible suggestions that you're surely going to
read here, I would like to point you to a paper I read a while ago in
Nature: In situ proteolysis for protein crystallization and structure
Hi Catherine,
At the Hauptman-Woodward Medical Research Institute High Throughput
Crystallization Screening laboratory we've just introduced SONICC and UV two
photon fluorescence into the imaging process. I don't think it's been announced
on the website
Dear All,
CCP4 releases new PISA web-server at the following URL:
http://www.ccp4.ac.uk/pisa . As always, constructive criticism, bug reports and
feature requests are welcome.
Enjoy!
Eugene Krissinel.
--
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A position is open for an Assistant Professor in the School of Chemical Biology
and Biotechnology, Peking University Shenzhen Graduate School. Currently SCBB
has structured its research and postgraduate education efforts around four
major units: Key Laboratory of Chemical Genomics, Laboratory
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