Dear Andreas,
maybe the problem is rather rooted in your expression set up. If the
protein was soluble in the small scale test, it is not necessarily the
case in a fermenter run. The parameters may have changed so strongly that
your protein is now expressed mostly insoluble. To my experience,
Andreas, you probably know all this, but I only understood quite recently.
What happens is that as ice crystals form you get brine rejection, the
same thing that happens in the arctic when sea water freezes. Therefore
you can have protein concentrated in pockets of high salt. Fine for some
Dear CCP4 members
I do not have any good experience with chemical cross linking, it has not
worked for me and I am curious how others have got it to work. In my latest
attempt I wished to crosslink two monomers with DSS. The protein is exclusively
dimeric according to SEC. I incubated the
FWIW for CCP4 we'd recommend 64-bit Linux.
Our 64-bit Linux version is downloaded now 6x more often than 32-bit
one. So it's more tested, and for some datasets pointless and aimless
require more than 4GB of memory (which is 32-bit limit).
Marcin
On Mon, Sep 29, 2014 at 02:05:32PM -0400, David
I take it you have the 32bit libraries installed. They are not normally
installed
by default. Then you need to make sure the app is linked with these 32bit
libraries.
I suspect that you may have linked to the 64-bit libraries.
Adam
Hi all,
So, I know this probably isn’t the right place,
Dear Careina,
First: make sure your solution is completely free of competing primary amines,
e.g. tris buffer. Phosphate buffers or HEPES should be used; if you need buffer
exchange, do it by gel filtration or extensive dialysis (2-3 changes), not
concentration-dilution.
Second: Try to use BS3,
Dear ccp4bb,
Could someone either provide, or point me to, a list of space-groups relevant
to protein crystallography just by space group number? I can find lots of
tables that list them by crystal system, lattice etc. but no simple list of
numbers.
Thanks,
Simon
Look at $CLIBD/syminfo.lib
All listed by number as well as other flags..
Eleanor
On 30 September 2014 13:17, Philip Kiser p...@case.edu wrote:
From XDS:
** LATTICE SYMMETRY IMPLICATED BY SPACE GROUP SYMMETRY **
BRAVAIS-POSSIBLE SPACE-GROUPS FOR PROTEIN CRYSTALS
TYPE
Be careful: the International Tables space group number may be ambiguous. For
example sg number 18 may refer to P 21 21 2 or its permuted settings P 21 2 21
or P 2 21 21, if you follow the proper IUCr convention that primitive
orthorhombic space groups have abc
The space group names are
Hi all,
Thanks for your help.
CORRECT.LP includes precisely the information I was after.
Also Ian Tickle’s article on http://www.ccp4.ac.uk/html/alternate_origins.html
is very helpful.
Simon
Thanks for all the suggestions. I think my issue is because I have a
QuadroFX1400 video card and I don’t have proper drivers - the generic ones just
won’t do it.
I have tried and failed miserably to install the proprietary drivers from
NVidea, so I’m not going to worry about it. I only use
Hi Dave,
the easiest way to install the proprietary Nvidia drivers on Fedora is
to use the RPM Fusion repositories.
The installation instructions for RPM Fusion are at
http://rpmfusion.org/Configuration.
Once the configuration files are installed you can install the Nvidia
driver as root on
The Biochemistry Molecular Biology Department at UMass Amherst is looking for
a tenure-track investigator. Please see the advertisement below.
Thanks,
Scott
Scott C. Garman, Ph.D.
Associate Professor
Department of Biochemistry and Molecular Biology
University of Massachusetts Amherst
1021K
Dear BB, or in particular Phaser developers :-)
This must be part of British humor right (or was that the Canadian influence
Randy) ?
eLLG indicates that placement of ensemble ensemble_1 will be straightforward
The data are sufficient to exceed the eLLG target
The search space is finite 143
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