Re: [ccp4bb] sodium thiocyanate as cryoprotectant

Hi All, Thank you very much for the suggestions! On Tue, Sep 27, 2016 at 4:37 PM, abhimanyu singh wrote: > Hi Alex, > > You may also think of gradually increasing PEG3350 concentration to around > 40% which has worked pretty well in some of my cases. > > Greetings, >

[ccp4bb] Latest XQuartz upgrade breaks Coot

More regarding" XQuartz upgrade breaks Coot” from people who know more than I do (which admittedly is most everyone, but I digress …): > > Today's Topics: > > 1. Xquartz 2.7.10-rc2 breakage (Jack Howarth) > 2. fink-0.41.0-101 du_sk.t build failure (Patrick Carmack) > > >

Re: [ccp4bb] "Core dumped" problem when running autoSHARP

Thank you all for information and suggestion. I have tried with stand-alone SHARP (meant with command line) but it yielded the same problem. For running on the SHARP server, I couldn´t get it work as it required the .htgroup file in the sushi configuration folder. Indeed, it worked with my boss

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Re: [ccp4bb] Latest XQuartz upgrade breaks Coot

Hi Logan, Thanks for this useful information. Didn't know it was possible to rejig binaries in that way! I'm taking the liberty of cc:ing your answer to the list. I forgot to say that this problem affected both the CCP4-distributed Coot and an old stand-alone from Bill Scott that I had in

[ccp4bb] AW: [ccp4bb] AW: [ccp4bb] output directory

Dear Eleanor, I am not that well organized, but we have a LIMS system that keeps track of the projects. To prevent mix-ups, I prefer not to work with different inhibitor complexes in the same directory, so I would have to create a separae ccp4i project for every complex. This is one of the

Re: [ccp4bb] AW: [ccp4bb] output directory

You must all be very well organised, and have a very good memory for where you ran that tidy-up job for some alternative project! Eleanor On 28 September 2016 at 07:51, wrote: > I fully agree. It would be great to be able to use the current working > directory

[ccp4bb] Latest XQuartz upgrade breaks Coot

Hi, I recently upgraded my Mac to Sierra (I know, early adopter...) and thereafter the latest XQuartz (2.7.10_rc3). The latter step seemed to break Coot, apparently because Coot requires the run-time library /opt/X11/lib/libXt.6.dylib and this has been replaced by /opt/X11/lib/libXt.7.dylib.

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Dear all, Please note the following opening : The School of Pharmaceutical Sciences at Xiamen University, China is seeking a highly motivated and talented protein crystallographer to join the faculty team. Job position will be commensurate with the candidate's qualifications.

[ccp4bb] AW: [ccp4bb] sodium thiocyanate as cryoprotectant

Dear Alex, Did you try freezing with the mother liquor as is? If you freeze fast enough, you may not need additional cryoprotectant with 25% PEG3350. You should first freeze some reservoir solution and look if it stays clear, before attempting to destroy a crystal. Best, Herman Von: CCP4