Dear CCP4 Community:
Happy Holidays!
Sorry for bringing up this old topic again. However after some recent
struggle with rather poor crystals and my deteriorating eye sights, I have
decided I need a couple 3D capable (cheap) workstations in lab.
After reviewing previous posts and the two
Hi,
Regarding the second publication below on the optimum solubility screen, I was
a new postdoc in Sung-Hou Kim's lab when the last parts of the work and
manuscript was being prepared, and so did quite a few experiments using the
screen in conjunction with DLS.
It is worth trying them out
Hi,
although most widely used, Tris-NaCl buffers may be acceptable for many
but unfortunately by far not all proteins.
The solubilty of a protein can be modulated by the use of the proper
anions and cations. This all depends on the pH of the solution and the
pI of the protein.
Consult the
