Hi Alex,
In addition to Mirella's suggestion I would like to make an addition which
might be specifically useful for you. Since your peptide has biotin tag, You
may use HABA dye assay for the exact quatifiation of biotin (and thus
biotinylated peptide). As far I recall, Thermo scientific
Hi Alex,
you can measure the absorbance at 214-220 nm, which is where the peptide bonds
absorb, but you should know/calculate/predict the extinction coefficient of
your peptide at that wavelength.
Furthermore, you might try BCA assay which is colorimetric as the bradford but
the reaction
Dear Pavel,
I believe words have a meaning, but they are not defined. This may make
languages a little more demanding than mathematics, since you have to deal
with a variety of a few hundred thousand to millions, depending how popular
the language in question is, but personally I enjoy the
Dear All,
Sorry for the off-topic question, I'd like to do Biacore SPR assay with
N-terminal biotinylated peptide as ligand (to Biacore SA chip) and my
protein as analyte. I have a question of how to determine the concentration
of biotinylated peptide （synthetic peptide), if the peptide has no
Hi Tim, hi Natesh,
one expression is mathematically, the other one is technically 'more
> correct'.
> I favour the terms poor and good resolution to avoid confusion, or
> explicitly
> list the values.
just out of curiosity.. what's your definition of 'poor' and 'good'
resolutions? I suspect
Dear John,
In protein crystallography, the ranges customarily applied to
resolution are:
Low resolution --> worse than 2.7 A
Medium resolution --> better than 2.7 A but worse than 1.8 A
High resolution --> better than 1.8 Aand worse than 1.2 A
Dear John,
one expression is mathematically, the other one is technically 'more correct'.
I favour the terms poor and good resolution to avoid confusion, or explicitly
list the values.
Best,
Tim
On Sunday, February 5, 2017 12:09:11 PM CET wrote:
> Dear All,
> For one protein crystal, its
It is confusing, but "high" is meant to indicate the quality of the
final electron density map based on the data. Your 1.8 A data set will
give the better map, and is the high resolution data set.
Dale Tronrud
On 2/5/2017 4:09 AM, wrote:
> Dear All,
>
> For one protein crystal, its
Dear All,
For one protein crystal, its resolution was 1.8 A. For another crystal for the
same protein, its resolution was 3.8 A. In literature, do we call the 1.8 A
crystal as the high resolution crystal (because of quality), or do we call the
3.8 A crystal as the high resolution crystal
