[ccp4bb] Postdoctoral fellow position available

Applicationsare invited for appointment as a postdoctoral fellow in the School ofBiomedical Sciences, the University of Hong Kong, to commence as soon aspossible for threeyears. Applicantsshould have a Ph.D. degree preferably in chromatin biology. Practical experience inmolecular and cell

Re: [ccp4bb] AW: [ccp4bb] Small molecule not refined in coot.

On 12/03/18 14:05, M T wrote: > Dear all, > > I restart this topic because the problem was finally not solved... You give me an opportunity to comment, I had previously missed the boat while traveling. > > Summary: > - I am working on a structure with an unnatural ligand and I want to > refine

[ccp4bb] PhD positions in "Structural Molecular Biology" at IISER Thiruvananthapuram.

Applications are invited from highly motivated students for admission to the PhD programme in the Structural Molecular Biology lab of Dr. Ramanathan Natesh in the area of Infectious Disease (*Mycobacterium tuberculosis* and *Plasmodium falciparum)

Re: [ccp4bb] Strange Diffraction pattern! Protein/DNA complex or DNA alone crystal?

The diffraction patterns clearly show an overlap of two or more lattices, which either means you didn’t have a single crystal at the start or it was damaged during the flash-cooling process. PEG 400 is generally a good cryoprotectant at ≥20%, but I would recommend using at least 25% to be

Re: [ccp4bb] Strange Diffraction pattern! Protein/DNA complex or DNA alone crystal?

Dear Xiao and Hans: Thanks for your reply. We tried to index it but failed. Joseph On Mon, Mar 12, 2018 at 11:15 PM, Xiao Lei wrote: > did you try to index it? the cell dimensions may give you hint. > > On Mon, Mar 12, 2018 at 4:40 AM, Joseph Ho

[ccp4bb] PhD and Postdoc Positions in Structural Biology

A PhD and a postdoc position in structural biology is available at the department of biochemistry at the Biocentre (head Prof. Utz Fischer) of the Julius Maximilians University, Wuerzburg/Germany in the field of RNP biochemistry. The successful applicant will establish protocols for the

[ccp4bb] New release of iMosflm (7.2.2)

There is a new release of iMosflm available from the MRC-LMB web site: https://www.mrc-lmb.cam.ac.uk/harry/imosflm/ The changes since the previous version are listed below, perhaps the most important is that this version will read the “full cbf”

Re: [ccp4bb] AW: [ccp4bb] Small molecule not refined in coot.

Dear all, I restart this topic because the problem was finally not solved... Summary: - I am working on a structure with an unnatural ligand and I want to refine this ligand using coot. - Each time I try to import the .cif of my ligand produced by PRODRG web server or through CCP4/ProDrg, coot

[ccp4bb] SEC cryo-EM

Hi all, I'm curious about which kind of chromatography set-up people are using for running size-exclusion chromatography as a final purification step prior to vitrification and cryo-EM. Especially for scarce samples/small volumes, you'd want to have a system with minimal dead volumes and the

Re: [ccp4bb] improper rotation matrix

I am struggling to see why one would want to apply an improper rotation, which I thought was defined as rotation plus reflection. Det=-1. Truly bad things happen to ones amino acids. Unless you had the coordinates from a small mol in one chiral arrangement, and wanted it in the other? But in

Re: [ccp4bb] improper rotation matrix

Assuming you have a good reason for this, you can apply any arbitrary transform fairly straightforwardly in ChimeraX. In the console (Tools/General/Shell), and assuming your model is the only one loaded: m = session.models.list()[0] import numpy transform_matrix = numpy.array([[r11, r12, r13,

Re: [ccp4bb] improper rotation matrix

Maybe you could turn your distorting matrix into into a fake symmetry operator? On 12 March 2018 at 10:24, Phil Evans wrote: > The command ROTATE in pdbset should allow to apply any valid rotation > matrix, ie determinant = +1.0 > > Anything else will distort the

Re: [ccp4bb] improper rotation matrix

The command ROTATE in pdbset should allow to apply any valid rotation matrix, ie determinant = +1.0 Anything else will distort the coordinates > On 12 Mar 2018, at 10:13, Franck Coste wrote: > > Hi all, > > I'd like to apply an improper rotation matrix to PDB

[ccp4bb] improper rotation matrix

Hi all, I'd like to apply an improper rotation matrix to PDB files but it seems it's not allowed in pdbset. Does anyone know a program where I could do this ? Thanks in advance. Regards, Franck. --