[ccp4bb] Are there reliable methods for Selenomet incorporation in Pichia ?

2018-03-28 Thread Anirban Banerjee
Dear all, There is not much by way of published literature on this. Are there unpublished methods that people have used and have found to work reliably ? Any help will be very much appreciated. Warm regards, Anirban

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Keller, Jacob
>>>These simple things are easily forgotten if there isn't direct visual >>>evidence for their existence. Maybe someday we scientists will collectively realize the huge importance of intuitive data presentation and focus more on it, scorn it less as "mere presentation"? When one thinks of the m

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Gerard Bricogne
Dear Jeffrey, Thank you for sharing your observations and the questions they bring up. I can see two things in the 3D displays for your two PDB entries, none of which seems new or surprising. The first is that there is a peppering of blue dots within the volume that otherwise seem

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Jeffrey B Bonanno
Hi Gerard, Very nice server indeed. I reviewed the 3D plots of some recent high res structures and am struck by the granularity which is not necessarily globally directional. For instance, I ran 4QRN and 5EHI and see "ripples" in the I/sigI plot between the various "layers" (higher res yellow t

[ccp4bb] Cryo-EM structures sought for CASP13

2018-03-28 Thread Cathy Lawson
Critical Assessment of Techniques for Protein Structure Prediction (CASP) community experiments aim to advance the state of the art in protein structure modeling. Every other year since 1994, CASP collects information on soon-to-be released experimental structures, passes on sequence data to the

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Keller, Jacob
>> I am not sure that there is any such thing as an up-to-date estimate of >> the prevalence of anisotropy in the PDB - but now you can get a feel for it >> yourself by looking at any entries you want. However please do not submit >> the whole PDB to the server - yet ;-) . It would be great

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Gerard Bricogne
Dear Jacob, Thank you for the appreciative comment. I am not sure that there is any such thing as an up-to-date estimate of the prevalence of anisotropy in the PDB - but now you can get a feel for it yourself by looking at any entries you want. However please do not submit the whol

Re: [ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Keller, Jacob
Wow, this is really cool--just tried a quick look at a recent membrane protein (5eqi) and you can see right away that there is anisotropy. I would guess this can be found in the literature, but how prevalent is anisotropy in the PDB? Jacob Keller +++

[ccp4bb] A new capability on the STARANISO server: "PDBpeep"

2018-03-28 Thread Gerard Bricogne
Dear all, Ever since the WebGL viewer became available on the STARANISO server, we have found ourselves using it with increasing frequency to take a quick look (a "peep") at the diffraction data deposited with various PDB entries - for example, to try and identify a root cause for some sub-op

[ccp4bb] PhD student position in Oulu, Finland

2018-03-28 Thread Lari Lehtiö
Dear all, We have a position open for a PhD student in the field of structural biology and drug discovery. The project focuses on structure and inhibition of ADP-ribosyltransferases. Information on our team and projects can be found from our web pages: http://www.oulu.fi/biocenter/groups/lehti

[ccp4bb] Open PhD positions at University of Montpellier, France – looking for a candidate

2018-03-28 Thread Laure Yatime
Dear colleagues, I am currently looking for a PhD student to join our research team at University of Montpellier (South of France) between September and December 2018. The PhD work will focus on the structural characterization of receptor-ligand complexes involved in inflammation during cance

[ccp4bb] Principal Beamline Scientist Positions at Diamond Light Source

2018-03-28 Thread David Hall
Dear all We currently have three vacancies to lead and develop three of our structural biology beamlines here at Diamond Light Source. Two MX beamlines: the in-situ serial crystallography beamline VMXi (http://www.diamond.ac.uk/Beamlines/Mx/VMXi.html): VMXi – http://www.diamond.ac.uk/Career

[ccp4bb] Beamline Scientist position at I24 Diamond

2018-03-28 Thread Robin Owen
Dear all, We have an opening for a beamline scientist at I24. I24 offers cryo, RT, in situ, and serial crystallography of microcrystals and this is an exciting opportunity to be at the cutting edge of microfocus and serial MX at Diamond. For more information and details on how to apply etc see