[ccp4bb] Professorship in Advanced Microscopy and Cellular Dynamics/Cryo-Electron Microscopy

2019-01-17 Thread Kristina Djinovic Carugo
Dear all, I would like to draw your attention to the opening of a full professorship in Advanced Microscopy and Cellular Dynamics/Cryo-Electron Microscopy in our Department (structbio.univie.ac.at) at Max F. Perutz Laboratories and University of Vienna. Please see the details below, closing d

Re: [ccp4bb] Partially occupied C-terminus

2019-01-17 Thread Wim Burmeister
Hello, can you do some ES-mass spectrometry on your sample ? There may be a truncated form in the sample and in this case the occupancy refined C-terminus would be a good solution. Otherwise I would invent an alternative conformation with 30 % occupancy and assign ver

[ccp4bb] Partially occupied C-terminus

2019-01-17 Thread Andreas Heine
Dear CCP4-BB, after refining a fairly high resolution aldose reductase structure (0.96 A) we observed negative Fo-Fc density (-3.0 sigma) for the three C-terminal residues (314-316). However, the 2Fo-Fc density clearly showed the position of these residues without indication of a second c

[ccp4bb] CCP-EM RELION 3 Image Processing Workshops 18th & 19th February 2019

2019-01-17 Thread Tom Burnley - UKRI STFC
For EM curious crystallographers we are running an introduction to Relion for EM image processing... Dear all, We are pleased to announce two one-day workshops introducing the basics of RELION 3 led by developer and structural biologist Rafael Fernandez-Leiro (Spanish National Cancer Centre,

Re: [ccp4bb] hybrid photon counter in the home lab

2019-01-17 Thread colin.n...@diamond.ac.uk
Jacob For some of these detectors, it is all quite complicated. See for example paper by Martin Stanton et al. https://www.sciencedirect.com/science/article/pii/0168900293904057 I am sure there are other analyses but this indicates the complexity Cheers Colin From: CCP4 bulletin board On Beha