Re: [ccp4bb] [g...@globalphasing.com: [ccp4bb] An error in the IUCr Online Dictionary of Crystallography] (fwd)

2020-03-10 Thread Gerard Bricogne
Dear Brian, Thank you for your reply. I think I could have provided you with the correct formula myself ;-) but it is indeed correct now. In any case, the verbal description I gave in my message specified it already. The definition in the mmCIF dictionary does look like the source of

Re: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine labeling

2020-03-10 Thread Bonsor, Daniel
Hopefully, someone will be able to share 293 Met- media. But if not, could you not use DMEM media with FBS, then deplete with DMEM with no glutamine, no methionine, no cysteine (thermos fisher 21013024) but supplemented with glutamax and cystine? The following paper describes it in the large

[ccp4bb] Expi 293 (Met-) expression medium for selenomethionine labeling

2020-03-10 Thread LAURA DEL AMO MAESTRO
Dear ccp4 community, I am in my last 3 months of my PhD, working at the Structural Biology Unit at IBMB-CSIC in Barcelona. As my experimental phasing trials using various heavy metals failed so far, we ordered two months ago Expi 293 (Met-) expression medium for selenomethionine labeling (see the

Re: [ccp4bb] AW: [EXTERNAL] [ccp4bb] An error in the IUCr Online Dictionary of Crystallography

2020-03-10 Thread Ian Tickle
It's a Wiki so anyone can edit it. I think you just have to create an account here: https://dictionary.iucr.org/index.php?title=Special:CreateAccount=Real-space+correlation+coefficient Note that you must have an entry in the World Directory of Crystallographers, otherwise your Username will be

[ccp4bb] AW: [EXTERNAL] [ccp4bb] An error in the IUCr Online Dictionary of Crystallography

2020-03-10 Thread Schreuder, Herman /DE
Dear Gerhard, By clicking on the "Actions" button, I discovered that the contribution comes from Brian McMahon. Maybe you could contact him about this? Best regards, Herman -Ursprüngliche Nachricht- Von: CCP4 bulletin board Im Auftrag von Gerard Bricogne Gesendet: Dienstag, 10. März

[ccp4bb] New Postdoc positions at the SGC in Oxford - Apply by March 16th

2020-03-10 Thread Opher Gileadi
Exiting postdoc positions for structural biologists to study new trug targets for Alzheimers and other neurodegenerative disease. Part of a large international consortium funded by the NIH, you will study the structure and function of diverse proteins, working in close cooperation with

[ccp4bb] An error in the IUCr Online Dictionary of Crystallography

2020-03-10 Thread Gerard Bricogne
Dear all, While we are all discussing how to "push the frontiers" of our field, there seem to be some dark and dusty corners in the documentation of some basic notions. For instance the formula for the real-space correlation coefficient given at

Re: [ccp4bb] Catalina compatibility

2020-03-10 Thread Oriana Fisher
Thanks! Sorry for bringing up something that was already discussed recently, but yes, that does still seem to do the trick (and in the process, after re-installing Xquartz, XDS is working as well). Thanks again, Oriana --- Oriana S. Fisher Assistant Professor of Chemistry Lehigh

Re: [ccp4bb] [3dem] Which resolution?

2020-03-10 Thread Kay Diederichs
I'd say that it depends on your state of knowledge, and on their I and sigma. - if you know the space group for sure before you do the measurement of the systematic absences, their I and sigma don't matter to you (because they don't influence your mental model of the experiment), so their