Re: [ccp4bb] number of frames to get a full dataset?

2020-06-25 Thread 00000c2488af9525-dmarc-request
Phil Jefferey was right about the point group (432), which looks like it represents about 0.7 % of the PDB! I tested the completeness with MOSFLM strategy for various sporadic missetting angles and 11 degrees of data does give you around 90 % completeness or more with redundancy close to 2,

Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Ginell, Stephan L.
Hi Pat, So sad to hear! I have seen such failures on the CX100 shipping dewars. Generally failure occurs close to the neck of the dewar and is apparently probably caused by a hard side or angular impact cracking a weld or the shell. Most the failures I have seen have been When shipping in the

Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Matthew Bowler
Dear All, just to add the perspective from the synchrotron side - these type of failures are actually extremely rare. Of course, this doesn't help if it is your crystals that have been thawed, but these stories should not dissuade you from shipping crystals. On MASSIF-1 at the ESRF all

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Jan Abendroth
Hi all, -apologies, hit send button too early- we have had very few dewar losses, even though we ship 2 dewars each weak to various synchrotrons. There might be a good element of luck, but we have implemented a couple measures that might help: - Most of our catastrophic losses happened with a

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Jan Abendroth
Hi all, we have had very few dewar losses, even though we ship 2 dewars each weak to various synchrotrons. There might be a significant element of luck, but we have implemented a couple measures that might help: - Most of our catastrophic losses happened with a break at the neck of the

[ccp4bb] Structural biology - Postdoc positions available

2020-06-25 Thread Demo, Gabriel
Hi everyone, I have two postdoc positions available in my newly established lab at the Central European Institute of Technology (CEITEC) Masaryk University Brno in Czech Republic. We are looking for candidates who are interested in determining structures of coupled viral-host

[ccp4bb] unable to launch ccp4 old interface on mac

2020-06-25 Thread Luca Mazzei
Dear CCP4 people, At the end of installation of CCP4 7.1 on mac OS Mojave, CCP4 folder contains ccp4i2 icon, whereas ccp4 icon is not present. This way I can not launch the old interface, neither by clicking on the invisible icon nor using the terminal. I just fixed the problem by a

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Syun-Ru Yeh
We had a similar experience with a CX100 a few years ago. We found some small chunks of white powder leaking out of the inner chamber of the dewar, which was associated with a dramatic reduction in the cooling capacity. We contacted a local distributor, who asked us to ship back the dewar for

[ccp4bb] Senior Scientist I, Protein X-ray Crystallography job opportunity

2020-06-25 Thread Nocek, Boguslaw P
Dear Colleagues, We have a job opportunity available for Senior Scientist I, protein X-ray crystallography in AbbVie (short description below). You can find more information and apply at: https://careers.abbvie.com/abbvie/jobs/2004610?lang=en-us=en-US Please forward it to anyone you know who

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Jan Dohnalek
The trouble is no other company does international LN2 shipments here ... So we stopped shipping dewars completely. Jan On Thu, Jun 25, 2020 at 2:27 PM Schreuder, Herman /DE < herman.schreu...@sanofi.com> wrote: > I had a similar one-sided discussion with FEDEX about their ignoring of > our

[ccp4bb] AW: [EXTERNAL] Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Schreuder, Herman /DE
I had a similar one-sided discussion with FEDEX about their ignoring of our customs declarations for Switzerland. That was then the last Dewar we sent by FEDEX. Best, Herman Von: CCP4 bulletin board Im Auftrag von Jan Dohnalek Gesendet: Donnerstag, 25. Juni 2020 08:34 An:

[ccp4bb] CryoEM Post-Doctoral Position

2020-06-25 Thread Hasan, Syed Saif
Interested in exploring the world of molecular espionage? Want to find out how viruses hitchhike between organelles during assembly? Join my lab as a post-doctoral researcher to decipher the secret life of epidemic causing viruses at the atomic scale. We use high-resolution single particle

Re: [ccp4bb] How many microfocus beamlines are in the world?

2020-06-25 Thread John R Helliwell
Dear Morpholino, In 1985 on a second generation synchrotron Xray source we, Daresbury SRS with Stanford SSRL staff, could mimic the on paper monochromatic undulator intensities of the proposed “New Rings“ of the 3rd generation with a focussed SRS white beam and thus showed that a gramicidin

Re: [ccp4bb] How many microfocus beamlines are in the world?

2020-06-25 Thread Andrew Leslie
Dear Morpholino, I think 10 microns or under is a reasonable way to define a micro focus beam line. There is a list of all MX beamlines at the following web site: http://biosync.rcsb.org/index.jsp This gives details of each beam line, including beam size,

Re: [ccp4bb] dewar horror stories

2020-06-25 Thread Jan Dohnalek
We have the suspicion (after several heavy FEDEX failures) they just toss them around ... then the neck easily breaks off. That only explains everything we have seen with completely damaged samples, lost, flying around the dewar etc ... When trying to communicate seriously with FEDEX about these