Re: [ccp4bb] Cell disruption

2020-08-15 Thread Pascal Egea
Dear Bernhard , I would recommend the emulsified from Avestin. It is great . Depending on your budget you can ask for stainless steel (probe to slow tear ) or ceramic (longer lasting but a bit more expensive). We have the ceramic but I have worked with the stainless steel one and it is really

[ccp4bb] Cell disruption

2020-08-15 Thread Bernhard Lechtenberg
Dear colleagues, We are currently looking to purchase a cell disruptor/homogeniser mainly for routinely processing a few 100 mls of E. coli suspensions. With the current COVID-19 restrictions it is very difficult for us to test any equipment. I thus hope that some of you can share their

Re: [ccp4bb] Does MicroED (microcrystal electron diffraction) have phase problem ?

2020-08-15 Thread Jessica Bruhn
Hi Alex, Welcome to the field of microED! From a practical standpoint, microED also suffers from the phase problem, and somewhat moreso compared to X-ray crystallography because anomalous signal is very limited. It is true that a TEM microscope operated in imaging mode produces images that

[ccp4bb] Does MicroED (microcrystal electron diffraction) have phase problem ?

2020-08-15 Thread Alex Lee
Hi, All, I am new to MicroED (microcrystal electron diffraction). I know that X-ray crystallography has phase problem, and I think MicroED has phase problem too (it is diffraction of electron instead of x-ray). However, when I read the Wikipedia, I could not understand the following description

Re: [ccp4bb] Homology modeling

2020-08-15 Thread Randy Read
Dear Amit, I don’t think that PROCHECK is the right tool for assessing the quality of a comparative model. It was revolutionary in its time, but the criteria it looks at can mostly be satisfied by energy minimisation even of an incorrect model, especially if you don’t have to simultaneously