Thanks for the suggestions.. actually I have been trying for quite some
already and this has lots of solvent channels ..hexagonal crystal with p62
group..not coming to a better resolution with completeness but am trying to
grow better and also trying to work out the data I already have
On Sun, 25
If the I/sigI and CC1/2 that you quote are for the outer resolution shell
of your data, you can use data to a higher resolution limit. It may still
be tricky, and rebuilding will be worse; as Eleanor says you'll probably be
better off growing a better crystal.
Good luck!
--
Kevin Jude, PhD
If you want to try co-crystallisation again, if you dissolve your ligand in say
DMSO or anything that works, e.g. isopropanol, then add it to your protein up
to its maximum tolerable level (i.e. the level up to which the protein is not
denatured/inactivated by the solvent - you need to test
You may soak the crystals with ligands in maximum tolerable DMSO percentage
and harvest crystals at different time points. In my experience working
with some poor affinity, highly hydrophobic compounds, it may take up-to
several days for the ligand to bind with good occupancy (in one instance it
How long did you wait before freezing the crystal? Sometimes I have to wait
days before the ligand finds it way into the binding site.
Casper Wilkens
Asst. Prof.
Structural Enzymology & Biorefining
DTU Bioengineering
Technical University of Denmark
