The Northwestern Feinberg School of Medicine is seeking a Protein Purification
and Crystallization Expert with an open rank for PostDoc, Research Associate,
or Senior Research Associate to participate in a Structural Genomics Research
Center addressing important biochemical studies related to
Maybe this will help?
https://bl831.als.lbl.gov/~jamesh/scripts/refmac_occupancy_setup.com
Make a pdb file of the residues you want to occupancy-refine and put it
on the command line of this script, along with the word "allatoms".
This will generate a file called "refmac_opts_occ.txt" that
We probably need more detail to help you.
Have you looked carefully at the data processing? Is the Rmerge or Rpim
reasonable for all batches? Is there any suggestion of twinning? Does the
wilson plot look linear? (These hexagonal SGs can be twinned)
How many copies of your molecule do you
Two postdoctoral positions are available in the team of Dr. Malene R. Jensen at
the Institute for Structural Biology in Grenoble, France.
The successful candidates will study the assembly of scaffolding complexes in
the mitogen-activated protein kinase (MAPK) cell signalling pathways. In
Dear Deepak,
I guess that the spacegroup in the MTZ file that you use for refinement is
wrong.
I think you should carefully check the output of PHASER, and in particular the
PDB file that it wrote. The correct spacegroup is given there (and it may also
be P4122 or P4222 or P4322, because
Dear CCP4 community,
I am refining several structures of multimeric protein-ligand complexes and I
wanted to refine occupancy of the ligand. Manual definition of groups would be
tedious and error prone considering that ASU contains 10 protein chains and 1-8
bound ligand molecules. Hence my
Hi folks,
A reminder: there is one week left to apply for this year's DLS/CCP4
workshop. Please see below for details.
Best wishes
-- David
On Tue, 6 Sept 2022 at 13:31, David Waterman wrote:
> We are pleased to announce that the 9th joint Diamond-CCP4 Workshop on MX
> data collection and
Dear all,
Greetings from Munich. I hope everything is well with you. I am writing to
take input on a problem related to the structure solution of a
protein-ligand complex.
I have crystallized a protein (7.6kDa) with a ligand (5.2kDa). The crystal
diffracted to *2.71 Angstron*, and the data were
Dear All,
Please suggest me a program to compute the pairwise CA-distance plots of
residues in other subunits after superposition of one particular subunit.
My enzyme is a homotetrameric one and upon binding of a substrate there are
changes in relative orientations among four subunits (i.e.,
