[ccp4bb] Äktaxpress uninet connector : Off topic

Dear All, Apologies for the off-topic post. We recently acquired some old Äktaxpress systems and learned that they require a specific USB to Uninet cable in order to connect to a PC. For now, we only find it on Cytiva for 2700 USD. Could you please guide me to some other seller that might have a

Re: [ccp4bb] Phaser 2.8.3: Hendrickson-Lattman coefficients generated from dataset lacking anomalous signal

Hi, Hendrickson-Lattman coefficients are just a way of storing phase probability information, and they can come from different sources including atomic models. Phaser puts in HL coefficients because they could be handy under some circumstances for combining the phase information from experiment

Re: [ccp4bb] Crystals with DNA

Hello Careina, In my hands, DNA protein complex crystals may be frustrating, because often we get good looking crystals which don't diffract at all and are actually not easy to improve. I remember obtaining a lot of crystal looking a bit like "STOP" road sign (octogonal shape for one axis) w

[ccp4bb] RES: [ccp4bb] Crystals with DNA

Hi Careina. The top two things that come to my mind is that you need to screen for different crystallization conditions and do some seeding. If your Crystal conditions does not diffract at all, probably it won’t. Have you tried to optimize your condintion changing the pH or precipitant concent

[ccp4bb] Phaser 2.8.3: Hendrickson-Lattman coefficients generated from dataset lacking anomalous signal

Dear all Is anomalous diffraction necessary for determining experimental phases and the Hendrickson-Lattman coefficients (HLA, HLB, HLC, and HLD)? MR solution from Phaser 2.8.3 (interfaced in ccp4 8.0.000 suite) seems to be generating HLA/B/C/D coefficients from an x-ray diffraction dataset. Wav

[ccp4bb] Cryo-EM Grant Program: Deadline March 31st 2024

Dear Community, You're invited to apply now to the Thermo Scientific Cryo-EM Grant Program and tell us how you plan to use cryo-EM methods to accelerate and advance your resea

[ccp4bb] Crystals with DNA

 Hello all. I am struggling to get defracting crystals with a protein DNA complex. The crystals are plentiful but they do not diffract. I am going back to the grind stone and relookong at my DNA sequence.Is there any wisdom you could give me with regards to what works best with DNA in crystals?F

[ccp4bb] Structural Biology Facility Manager vacancy at the John Innes Centre, Norwich UK

Dear All, We have a vacancy for a Structural Biology Facility Manager at the John Innes Centre. The John Innes Centre is an independent, international centre of excellence in plant and microbial sciences. We nurture a creative, curiosity-led approach to answering important questions in bioscie