Re: [ccp4bb] mtz/cif file for depostion

From your refmac refinement job in i2, this should give you the combined mtz? [test.jpg] Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent with [Proton Mail](https://proton.me/) secure email. On Friday, 9 February 2024 at 17:54, Palm, Gottfried

Re: [ccp4bb] mtz/cif file for depostion

Hello, there's a way to export all the columns in one file, rather than trying to deposit two mini-mtz's. The problem is remembering how when I'm away from the computer. It's one of the export options that you find when digging around a bit. Maybe it needs a right-click on one of the icons -

[ccp4bb] mtz/cif file for depostion

Dear all,   I have problems preparing my experimental data (XRD) for deposition. I have two mtz files, which work well for refmac5, with an FREER column in one file and Iplus, SIGIplus, Iminus, SIGIminus columns in the second file. I merged them into one mtz file with cad (in ccp4i). The

[ccp4bb] R: [ccp4bb] how to use electron scattering factors in REFMAC program?

thank you, Robert. simpler than expected. I'll try it right away Dedi Da: Roberto Steiner Inviato: venerdì 9 febbraio 2024 10:45 A: BENEDETTA CARROZZINI Cc: Adedunni Adenuga Oggetto: Re: [ccp4bb] how to use electron scattering factors in REFMAC program? Hi

[ccp4bb] R and TechDev Funding Calls Open for 2024

Dear all, This is a reminder to apply for the Instruct-ERIC R Pilot Call, and brand new TechDev Call! R Funding Call The Instruct-ERIC R Funding Call for 2024 is now open. Early career researchers are invited to submit proposals to access Instruct technology, equipment, and expertise

Re: [ccp4bb] Crystals with DNA

Carina, to complement the techniques using sticky ends, etc., you can also use the "random" microseeding approach that I mentioned to Kavya, see below. The great advantage in a project like yours, where you have a family of related constructs, is that you can use cross-seeding - that is, you can

Re: [ccp4bb] Phaser 2.8.3: Hendrickson-Lattman coefficients generated from dataset lacking anomalous signal

If I remember for coefficients with FOM and a precise PHI calculated, A = FOM*cos(phi) B = FOM*sin(phi) C=D=0 If you have a probability curve for PHI. 0 to 360 such as you get from experimental phasing, A B C D mirror this bimodal curve better .. On Fri, 9 Feb 2024 at 09:58, Nitin Kulhar <

Re: [ccp4bb] Crystals with DNA

Hello, Overhanging "sticky" ends are mentioned frequently when it comes to obtaining infinite helices that are useful in crystallization. For example in https://home.ccr.cancer.gov/csb/nihxray/Tips-and-Tricks_Crystallization_Protein-DNA_updated.pdf . Cheers, Fred. On 09/02/2024 10:59,

Re: [ccp4bb] Crystals with DNA

Hello, If you want to promote "infinite" helix, you should go for overhangs (sticky end)  with compatible sequence. I try to explain better what I have in mind (and I actually did). example : 5'ATCCCTAAATCGGCGTGTGCT---3' 3'---GGATTTAGCCGCACACGATAG5' Hoping that this results in something

Re: [ccp4bb] Crystals with DNA

Thank you for this insight, Nicolas. It is very helpful.Yes I have also had a soccer ball shaped crystal that does not diffract as well as, and more recently, many plate like crystals but they do not diffract either.I do know I have both protein and DNA in my crystals but I do not know, as you

Re: [ccp4bb] Phaser 2.8.3: Hendrickson-Lattman coefficients generated from dataset lacking anomalous signal

Dear sir Thank you for clearing that. I checked back to see that HLC/D are invariably 0 for all reflections, with the non-zero HLA/B supposedly having been originated from the probability distribution of phases *calculated* by phaser. Hopefully, I have not misunderstood it. Thanks and regards

Re: [ccp4bb] how to use electron scattering factors in REFMAC program?

Hi Benedetta Use an external keyword file that with the line 'source ec mb’ Best R Roberto A Steiner www.steinerlab.org https://twitter.com/steiner_lab roberto.stei...@kcl.ac.uk Randall Centre for Cell and Molecular Biophysics

[ccp4bb] how to use electron scattering factors in REFMAC program?

Dear CCP4BB community, I am working on the structural solution of macromolecules via MR using 3D electron diffraction data. I would like to know if it is possible to carry out a preliminary refinement (under 'kinematic' conditions) of the MR model with REFMAC. If so, what are the options (or