[ccp4bb] PostDoctoral Position in GPCR Structural Biology at USC, Los Angeles

2024-04-17 Thread Cornelius Gati
PostDoctoral Position in GPCR Structural Biology at USC, Los Angeles The position is available starting immediately in the Department of Biological Sciences at the University of Southern California and is initially limited to 2 years, with possible extension. Our research focuses on the

Re: [ccp4bb] Rescale merged data?

2024-04-17 Thread Robbie Joosten
If I may add to that: Please deposit the full dataset, not just the set of reflections you end up using. This allows people to use all the data if they are interested.Cheers,RobbieOn 17 Apr 2024 22:35, "Hekstra, Doeke Romke" wrote: Hi Matt,   I appreciate disagreement and comments from

Re: [ccp4bb] Rescale merged data?

2024-04-17 Thread Matt Mcleod
Thanks Doeke (and others who have replied directly). I suppose my big concern with just truncating the data after merging would be the bin size/data processing statistics. If the edge/corner is binned 10x and I end up removing 5 of the bins by truncating the resolution post-merging, the Table 1

Re: [ccp4bb] Rescale merged data?

2024-04-17 Thread Hekstra, Doeke Romke
Hi Matt, I appreciate disagreement and comments from colleagues. My two cents are that it seems unnecessary to repeat scaling and merging, or any earlier step. If you want to remove structure factor amplitudes or merged intensities from the MTZ file you can do so using MTZUTILS or similar

Re: [ccp4bb] Rescale merged data?

2024-04-17 Thread Matt Mcleod
Sure thing. A former student left somewhere between 30-50 datasets but they scaled the data to the detector corners (or maybe edge) in HKL2000. There are many of the high-resolution bins with no reflections in them. He then went forward and merged this data, presumably in HKL2000 again and did

Re: [ccp4bb] Rescale merged data?

2024-04-17 Thread MyeongSeon Lee
L pm xzx Have a grea lol On Wednesday, April 17, 2024, 1:59 PM, Hekstra, Doeke Romke wrote: Hi Matt, It would be helpful if you could describe your case in more detail. Do you want to change the resolution cutoff after scaling? Do you want to keep more data? Fewer? Or do you mean something

Re: [ccp4bb] Rescale merged data?

2024-04-17 Thread Hekstra, Doeke Romke
Hi Matt, It would be helpful if you could describe your case in more detail. Do you want to change the resolution cutoff after scaling? Do you want to keep more data? Fewer? Or do you mean something different such as truncation to generate amplitudes, application of anisotropic resolution

[ccp4bb] Rescale merged data?

2024-04-17 Thread Matt McLeod
Hi all, I am looking at a old students data and it looks like they didn't properly cut off the data during scaling. All of the files I have appear to be the merged .sca (or mtz after converting with scalepacktomtz) - is there a way to retruncate the data after merging or do I have to

[ccp4bb] ECA Prizes open for nomination

2024-04-17 Thread Isabel Uson
Dear Colleages, I draw to your attention the following announcement from Arie van der Lee, ECA vicepresident, as there are new, less well known additions. An education prize in the name of Lodovico da Riva Sanseverino, of whom so many of us keep fond and grateful memories for the role the Erice

Re: [ccp4bb] Request for assistance with structure solution

2024-04-17 Thread Kay Diederichs
good detective work indeed! Kay On Tue, 16 Apr 2024 14:40:15 +0100, Yehudi BLOCH wrote: >Dear Adewumi > >I'm afraid the 85% complete dataset is a contaminant as well. >PDB 2r6s and related entries for E.coli Gab protein have near identical unit >cell parameters. The autoprocessed (?) .mtz