Re: [ccp4bb] Trimming of carbohydrate chains

Dear Herman, Assuming that the protein is eukaryotic, and glycans N-linked, various ways of dealing with the issue are described in PMID: 17355862. Best wishes, radu -- A. Radu Aricescu, PhD MRC Senior Research Fellow Associate Professor University

Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!

or more cells in the field? This does not look like transient expression using a normal plasmid... Very intriguing nevertheless! radu -- A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Human Genetics Division

[ccp4bb] Postdoctoral position, C2D2, University of York

-Discipline-hopping-internships or http://www.york.ac.uk/c2d2/internships/ For informal enquiries contact: radu.caline...@york.ac.uk -- A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Human Genetics

Re: [ccp4bb] install crystallography software on mac

Best place to start: http://scottlab.ucsc.edu/~wgscott/xtal/wiki/index.php/Crystallography_on_OS_X -- A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology

Re: [ccp4bb] The effect of His-tag location on crystallization

Or indeed support surreal structures :-)) (PMID: 11853672 vs PMID: 14749821 and so on) -- A. Radu Aricescu, PhD University Research Lecturer MRC Career Development Award Fellow University of Oxford Wellcome Trust Centre for Human Genetics Division

Re: [ccp4bb] mammalian expression vector

Hi Jerry, You may find that pcDNA3.1 won't give you the protein yields needed for crystallization. Have a look at PMID: 17001101 for an alternative. Your Kozak sequence looks good, radu -- A. Radu Aricescu, PhD University Research Lecturer MRC Career

Re: [ccp4bb] FW: [ccp4bb] highly glycosylated protein

, play with deglycosylation conditions etc) and produce a more homogeneous sample than CHOs (which are leaky, see Chang et al 2007, PMID: 17355862, for details). Best wishes, radu -- A. Radu Aricescu, PhD University Research Lecturer MRC Career

Re: [ccp4bb] Deglycosylation enzymes

not a bad deal in long term. Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom Phone: +44-1865-287564 Fax: +44-1865-287547

Re: [ccp4bb] update on SeMet production

others followed. More recently, people have moved to transient expression and probably the easiest labelling method for HEK293 cells can be found in PMID: 17001101. Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre

[ccp4bb] OXION Postdoc fellowships and DPhil/PhD studentships

expression, purification and crystallization are warmly invited to apply :-) Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom

Re: [ccp4bb] Deglycosylation

design (eliminate Ser/Thr/Pro-rich domains), for other types have a look at http://www.prozyme.com/pdf/gk80110.pdf for example. Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division

[ccp4bb] Fwd: [ccp4bb] HEK293S

Dear Vaheh, I'm not aware of a commercial or cell bank source, but you can get these cells from HG Khorana's lab at MIT. Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural

Re: [ccp4bb] insect expression system

Se-Met labelling and so on. Here are some references (PMID): 17355862, 17001101, 16823037, 11788735, 16082028. Radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive