Oxalate. Sometimes forms when PEGs get old and begin to oxidize. That's some
of the stuff that is responsible for PEG's becoming progressively more
acidic as they age.
No idea if you have PEG in solution or not.
Alternative - CH3-C(=O)-COOH
Disordered dimethyl formamide
Dimethyl acetamide
If you're worried about UV all you need to do is to run multiple lanes of
the same stuff, shield all but one with some sort of UV-opaque material
(many kinds of plastics work very well), and cut the shielded lanes based on
what you see on the illuminated lane. In a properly cast gel, all the lanes
And obviously, each crystallography lab should invest in a Wii station with
a full complement of games - for training purposes.
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Stephen Graham
Sent: Thursday, June 21, 2007 10:46 AM
To:
Hi,
In my opinion this is clearly a twinned crystal of some sort of a small
molecule. If you have a small ice crystal on top of a completely
non-diffractive protein crystal, sometimes the pattern may also look like
this - but I would bet this is just 'salt'.
Artem
_
From: CCP4
You have to judge using some sort of a biochemical or biophysical method.
Yes, protein can be functionally/structurally impaired and yet still soluble
- however there is very little reason to suspect this due to salt exposure -
unless you have hard evidence.
I have worked with several proteins
Dear Yong,
There are several MS-specific programs that I know of but they're not in the
public domain. I have a couple of crude PERL scripts for this and an Excel
application written by a former colleague. These are too crude to send out,
but I would be happy to run your sequences and masses
What often works for this scenario:
Change temperature. Try 21, 15, and 4C.
Add glycerol or ethylene glycol - set a screen with your condition as basis,
and % EG/glycerol across - from 1% to 15% in suitable increments
Find additives that produce no crystals, set up tray(s) with those, then
Hi Dima,
Can anyone comment on availability of Archaea expression systems?
Based on available structures, it would appear that their chaperons
are much more similar to eukaryotic one than anything present in
eubacteria. For a long time, I've been puzzling over why there is
still no
Weijun,
There are literally hundreds of options you can pursue - obviously a
comprehensive and detailed list of suggestions would not fit in a little
email (in fact they don't all fit into a one-volume book).
It would be helpful to narrow things down a bit - for starters, for one or
two cases
Its much better to refer to your machinery by its first name they work
better that way. My AKTA machines always had Teutonic names: Helga,
Brunhilde, Gertrude, just to name the last three.
Artem
_
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Derek
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