What often works for this scenario:
Change temperature. Try 21, 15, and 4C. Add glycerol or ethylene glycol - set a screen with your condition as basis, and % EG/glycerol across - from 1% to 15% in suitable increments Find additives that produce no crystals, set up tray(s) with those, then seed with your microcrystals (worked really well for me in a few cases) Reduce protein concentration Add gelling agents to the set-up Regards, Artem _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Jobichen Chacko Sent: Thursday, March 29, 2007 7:45 PM To: [email protected] Subject: [ccp4bb] Multiple nucleation Hai All, Sorry for the non Ccp4 question. I got very small crystals for a protein and I am trying to optimize the condition to improve the size of the crystal and reduce the number of nucleation. The crystals are coming from five to six conditions , all have PEG 3350 in common. Apart from PEG, the condition has Lithium sulphate and Bis Tris in one condition and Ammonium suphate and HEPES in another condition, while the third condition is Sodium malonate, Bis Tris and PEG. I am getting thousands of small crystals with some precipitation. I tried macroseeding and additive screen, but the crystals are not growing. Any suggestions are more than welcome. Thank you. Jobi
