Re: [ccp4bb] Easy/Silly question

2022-07-01 Thread Casper Wilkens
Hello, You can build it from the two monomers in Coot. All the best, Casper From: CCP4 bulletin board On Behalf Of Paul Emsley Sent: 1. juli 2022 06:17 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Easy/Silly question On 01/07/2022 04:50, Joel Tyndall wrote: Hi folks, I frustratingly

[ccp4bb] Sv: [ccp4bb] MR solution not working

2022-03-02 Thread Casper Wilkens
Are the mutant and wild-type in the same space group and are the unit cell dimensions similar? If so, a simple rigid body refinement could work. Fra: CCP4 bulletin board på vegne af Shubhashish Chakraborty <750c9a1ca48b-dmarc-requ...@jiscmail.ac.uk> Sendt:

[ccp4bb] Sv: [ccp4bb] Strategies for increasing Trp content of proteins

2022-02-19 Thread Casper Wilkens
Is absorbance at 205 nm not a possibility, Jack? Casper Wilkens Asst. Prof. Structural Enzymology & Biorefining DTU Bioengineering Technical University of Denmark [http://www.dtu.dk/%7E/media/DTU_Generelt/Andet/DTU_email_logo_01.gif] Department of Biotechnology and Biomedi

[ccp4bb] Job posting - Post Doc in polysaccharide lyase mechanisms

2021-07-31 Thread Casper Wilkens
is August 27. For more information and link to the online application form see: https://www.bioengineering.dtu.dk/english/about/jobliste/job?id=ce47a7c3-5982-4045-9801-28020ff0a197 All the best, Casper [http://www.dtu.dk/-/media/DTU_Generelt/Andet/mail-signature-logo.png] Casper Wilkens Assistant

[ccp4bb] Sv: [ccp4bb] Co crystalization with less soluble ligand.

2021-04-24 Thread Casper Wilkens
How long did you wait before freezing the crystal? Sometimes I have to wait days before the ligand finds it way into the binding site. Casper Wilkens Asst. Prof. Structural Enzymology & Biorefining DTU Bioengineering Technical University of Denmark [http://www.dtu.dk/%7E/m