[ccp4bb] Biological Chemistry Position at UWA (Perth, Australia) - Brian Metcalf Chair

, Charlie -- I work flexibly and I am sending this message now because it suits me. I don’t expect that you will read, respond, or take action outside of your normal working hours! Charlie Bond (he/him) – http://bondxray.org<http://bondxray.org/>/ Professor, FRSC GAICD, Ally School of Mol

[ccp4bb] CryoTEM specialist wanted in sunny location

Dear colleagues, Our microscopy facility is installing a new cryoEM suite and The University of Western Australia is urgently looking for a cryoTEM specialist! CryoTEM specialist Exciting opportunity for a passionate scientist with cryoTEM experience to work in a multi-disciplinary research

Re: [ccp4bb] Unusual monomer-monomer interface in crystal

repeated the experiment to get better data, on crystals from the same tray, and the nickel had transmuted into zinc. Zinc had not been added on purpose at any point. Cheers, Charlie -- Charlie Bond – http://bondxray.org<http://bondxray.org/>/ School of Molecular Sciences https://www.uwa.

Re: [ccp4bb] Does anyone have a copy of the legacy O symmetry files

Excellent – I have had tarball-containing responses from 3 fantastic people Robert Esnouf, Stefano Trapani and Martin Martinez Ripoli. I will have a look at what they sent and hopefully will find what I need. Many thanks all. Cheers, Charlie -- Charlie Bond – http://bondxray.org<h

[ccp4bb] Does anyone have a copy of the legacy O symmetry files

lso disappears at the FTP server. If anyone still has their old O installation in place and can send me the files I will be very grateful. Cheers, Charlie -- Charlie Bond – http://bondxray.org<http://bondxray.org/>/ Head, School of Molecular Sciences https://www.uwa.edu.au/science/home/schoo

Re: [ccp4bb] Alignment software

, Mohd -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406

Re: [ccp4bb] Protein topology plot

. The server for Topdraw seems not to work anymore. -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406

Re: [ccp4bb] Mounting needle-shaped crystals

recommend Mitegen MicroMeshes or another tool for mounting needles? Thanks, Jack Tanner -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488

Re: [ccp4bb] force display of sheet in PYMOL

sheet. Is there any way? Thanking you in advance... Raja Try the new Yahoo! India Homepage. Click here http://in.rd.yahoo.com/tagline_metro_1/*http://in.yahoo.com/trynew. -- Charlie Bond Professorial Fellow University

Re: [ccp4bb] backbone b-factor

.* Mayo Clinic Cancer Center Griffin Cancer Research Building 4500 San Pablo Road Jacksonville, FL 32224 Tel:(904) 953-0046 Fax:(904) 953-0277 *salameh.m...@mayo.edu* -- Charlie Bond Professorial Fellow University of Western Australia

Re: [ccp4bb] is my crystal twinned or not?

for delivering it to the intended recipient, you are hereby notified that reading it is strictly prohibited. If you have received this e-mail in error, please immediately return it to the sender and delete it from your system. Thank you. -- Charlie Bond Professorial Fellow University of Western

Re: [ccp4bb] Self Rotation map in R32?

- Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D 8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35

Re: [ccp4bb] Density for pyrophosphate?

at 3 sigma). I have also tried to fit in the density as two free phosphates, and the results are worse. Can I get some advices on this? Any suggestion will be greatly appreciated. Best regards, Junyu -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical

Re: [ccp4bb] multi-domain protein with identical tertiary structure

multi-domain protein (with at least two domains) which has identical tertiary structure of each domain ? Thanking you. -regards shankar -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley

Re: [ccp4bb] TEV nucleotude sequence with restriction site

*** Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu *** -- Charlie Bond Professorial

Re: [ccp4bb] Pymol

of residues and showing them as a strand or helix. Thanks for helping. Mariah -- Mariah Jones Department of Biochemistry University of Florida -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway

Re: [ccp4bb] Offtopic: Alscript question

, Cornell University Ithaca, NY 14853 === -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406

Re: [ccp4bb] Phasing at Low Resolution

/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 RNA is really nifty DNA is over fifty We have put them both in one book Please do take a really good look http://www.rsc.org/shop/books/2008/9780854042722.asp -- Charlie Bond Professorial Fellow University of Western Australia School

[ccp4bb] BioRad CFX96 for thermofluor/DSF?

here had any advice. I'll summarise any responses I get. Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406

Re: [ccp4bb] Calculation of angle between two helix of different subunits

product' or something similar). Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310,  35 Stirling Highway Crawley WA 6009, Australia

Re: [ccp4bb] Manganese buffers for protein crystallization

reduced the pH (transferred crystals to new drops) and they survived to the point of pH7 where we supplemented with Mn2+. Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway

Re: [ccp4bb] Angle between two different helices from two different subunits

mailto:cedric.bauv...@ulb.ac.be tél: +32 (0)2 5273634 fax: +32 (0)2 5267273 .. -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009

Re: [ccp4bb] Could I improve an inconsecutive experimental map?

~ :) -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310,  35 Stirling Highway Crawley WA 6009, Australia

Re: [ccp4bb] Off-topic: ligand enrichment

://www.ncbi.nlm.nih.gov/pubmed/15102853?dopt=Abstract Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406

Re: [ccp4bb] Choosing MR solutions in the case of perfect twinning with P41212?

78FD 6669 67BA 8D5D -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia charles.b...@uwa.edu.au +61 8 6488 4406

Re: [ccp4bb] crystallisation and mosaicity

these problems. Thankz Sajid From Chandigarh to Chennai - find friends all over India. Go to http://in.promos.yahoo.com/groups/citygroups/ . -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35

Re: [ccp4bb] Are Calcium Citrate Crystals A Common False Positive?

for crystals in the reservoir is another 'control'. Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia [EMAIL PROTECTED] +61 8 6488 4406

Re: [ccp4bb] Rant: B vs TLS, anisou, and PDB headers

the structure, then that's a problem we have to solve in other ways. Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia [EMAIL PROTECTED] +61 8 6488 4406

Re: [ccp4bb] rescuing crashing-out protein eluted from Nickel column

a matter of minutes. I felt so helpless, didn't know what to do, and then decided to add 5% of glycerol into one of the fractions but that made it even more cloudy (ohh no...). While the protein is dying in the tube, do you have some quick remedy for me? Thanks very much, -J.J. -- Charlie Bond

Re: [ccp4bb] Real space rotation translation

of describing a conformational change by measuring the rotational and translational displacement of a domain from a starting structure. Thank you, Ben B . -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling

Re: [ccp4bb] Sulfate ion on 2-fold axis

and two O atoms with occu=1, or should I put one S and four O atoms all with occu=0.5? Thanks for your inputs. Jie . -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009

Re: [ccp4bb] Twinning: P43 apparently P42212

as many atoms per au). Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia [EMAIL PROTECTED] +61 8 6488 4406

[ccp4bb] Twinning: P43 apparently P42212

is how we see 00l=2n reflections for a structure that is apparently P43. Any explanation of this phenomenon and why it may contribute to the R-factor not going down despite excellent, informative, atomic electron density would be gratefully received. Cheers, Charlie -- Charlie Bond

Re: [ccp4bb] MSE

Kim Henrick wrote: A standard amino acid is a gene product with a specific t-RNA. Isn't it arguable that Met-tRNA is no more specific for S-Met than for Se-Met, it's just a matter of what the organism has been eating? Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western

Re: [ccp4bb] Survey on computer usage in crystallography

you know nothing about yet, or are comparing superpositions of a clutch of loosely-related structures, stereo can be an extremely useful. Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310, 35 Stirling

Re: [ccp4bb] Program to find the center of mass

Just to complete the set, in pdb-mode for emacs, if you do pdb-increment-centroid 0 0 0 (e.g. to move a molecule to the origin), it reports the original centroid. Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular

Re: [ccp4bb] CCP4 GUI

the refine temperature factors box. However, when I look at the resulting pdb file my B factors vary from 2 to 90. Is Refmac just calculating my B factors or is it still refining them, and if the latter how can I stop it? Thanks, Simon -- Charlie Bond Professorial Fellow

Re: [ccp4bb] Expression hosts

is usually a problem). Have a look at http://www-users.york.ac.uk/~jpjc1/facilities.htm . Cheers, Charlie -- Charlie Bond Professorial Fellow University of Western Australia School of Biomedical, Biomolecular and Chemical Sciences M310 35 Stirling Highway Crawley WA 6009 Australia [EMAIL