But, how with x-rays can one experimentally tell the difference between a
hydrogen and a filled orbital (say of N)? I will grant that the electron
density for a bound H should extend farther from the heavy atom but I believe
you would need resolution better than 0.89 Ang to see this difference.
1. We like Bugbuster. You can get it at 10X concentration so it is
fairly economical. We do add lysozyme and DNase as well.
2. We use the NuPage gels and SimplyBlue SafeStain and it works very
well. We can run a gel in 20-30 min and have it stained and destained a hour
later using the
Dialing,
Malonic acid is dissolved in water and then pH adjusted to the desired value
with NaOH. Caution: dissolving malonic acid is highly exothermic. Do it slowly,
in a hood.
Doug
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Dialing
Pretty
Sent: Wednesday,
concentration. If you just remove the PEG without washing it will
continue to concentrate.
Doug Ohlendorf
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of rashmi
panigrahi
Sent: Saturday, January 28, 2012 9:55 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] protein lost
Related to manual indexing, 20 years ago I wrote a program to transform a
list of peaks from the original Xentronics detector to points in reciprocal
space. The peaks were written as a PDB file so the peaks (now waters) could
be displayed on any graphics program. It was very useful for seeing and
Frances
It looks like the 3-fold is combining with dyads to make a pseudo 432
arrangement. If you're of space group and that there is only one trimer in
asymmetric unit, look internal structural similarity within a monomer. For
instance, multiple similarly folded domains.
Doug Ohlendorf
Tim,
Not really. Fo's can be viewed as having 3 parts. 1) F from our modeled
structure, 2) F from what we can't model. This can be bulk solvent, partial
or multiple occupancies in low resolution structures, thermal anisotropy ,
etc. 3) F from random errors in measuring data. The first F is what
Andy,
One important thing if your complex crystals are isomorphous with apo is to
use nFo(complex) - Fo(apo). These maps give the maximum information as the
uninterpretable 'stuff' in the Fo-Fc map is likely quite similar in both
crystal forms so the difference signal should be cleaner.
Douglas
:
http://biosci.cbs.umn.edu/bmbb/ohlen_lab/index.html
-Original Message-
From: Ian Tickle [mailto:i.tic...@astex-therapeutics.com]
Sent: Wednesday, June 17, 2009 12:08 PM
To: Doug Ohlendorf
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: RE: [ccp4bb] Difference Map images
Hi Douglas
Do you have
