Re: [ccp4bb] Difficult Molecular replacement

Hello Marc- My first thought is do you have the correct space group? Are you searching for other space groups in the point group? Second, helicases are multimers, but the orientation of the monomers that make up the multimers may deviate significantly. If you didn't use a monomer, it's worth

Re: [ccp4bb] Database for submitting unprocessed diffraction data

Two other choices: https://data.sbgrid.org/ https://proteindiffraction.org/ Best- Todd From: CCP4 bulletin board on behalf of JEROME JOHNSON Sent: Wednesday, October 18, 2023 11:23 AM To: CCP4BB@JISCMAIL.AC.UK

Re: [ccp4bb] Semet derivative dying almost immediately in beam

Certainly not trying to be insulting by the suggestions but could it be as simple an alignment issue with the crystal, issues with the beam alignment or crystal damage following a fluorescence scan? The cryo setup? Like James, I’ve seen crystals decay quickly in high salts at longer

Re: [ccp4bb] Questionable Ligand Density: 6MO0, 6MO1, 6MO2

I like cases like this as cautionary tails for the crystallography class. In this case, the sequence is mismatch in several places, ex. residues 1165-1170 should be 1153-1158 which flows nicely after you add a residue to 1151. There are other places where the mainchain has slid and is out of

[ccp4bb] programs to representation of common surface feature from a series of PDB files

Hello All- I am overlaying a series of structures that have a similar sequences and the same topology, and I would like to represent an illustration to shown commonality or lack thereof across the surface of the protein. I am most interested in surface, ie. differences in peaks and valleys.

Re: [ccp4bb] AW: Re: AW: [ccp4bb] tNCS problem

On a side note, I assume that you have searched with both P2 as well as P21 for your space group. If not, I will point out that there are 265 submissions in the rcsb for the P2 space group, compared to 20,698 for P21. That makes P2 fairly rare. Good Luck! - Todd