Re: [ccp4bb] analytical gel filtration columns

2019-04-23 Thread Mohinder Pal
, Mohinder Pal -- "Whatever you’re meant to do, do it now. The conditions are always impossible.” Doris Lessing -- > On 18 Apr 2019, at 07:36, Bärbel Blaum wrote: > > Hi Mohinder, > > if

Re: [ccp4bb] analytical gel filtration columns

2019-04-17 Thread Mohinder Pal
for better resolution as I plan to add more components to this existing complex. Best wishes, Mohinder Pal -- "Whatever you’re meant to do, do it now. The conditions are always impossible.” Doris Le

Re: [ccp4bb] suggestion of crystallization optimization

2018-06-04 Thread Mohinder Pal
Dear Liuqing, It could be good to dissolve the crystals to check if the crystallised protein is still intact. If it is truncated then make a new construct based on truncation. It could improve the crystal diffraction as it was the case for me. Best wishes, Mohinder

Re: [ccp4bb] His-6 versus His-10 tag

2017-09-19 Thread Mohinder Pal
Dear Herman, I have crystallised the same protein with 6xHis and 12xHis and they both diffracted to high resolution. However, I have not seen these tags in the structure. Best wishes, Mohinder Sent from my iPhone > On 19 Sep 2017, at 12:10, herman.schreu...@sanofi.com wrote: > > Dear BB,

[ccp4bb] Regarding space group P1, P21

2010-10-21 Thread Mohinder Pal
structure but I am just curious to know one restrain equals how many observations. I look forward to hear your suggestions. Kind regards, Mohinder Pal

[ccp4bb] Regarding CCP4 on mac

2009-09-16 Thread Mohinder Pal
Dear ccp4bb, I have just installed ccp4 6.1.2 with ccp4i 1.4.2 on a MacBook Pro running OSX 10.5.8. When I try to run Refmac5 (or phaser) I get the status starting in the GUI but the program fails to run and no log file is generated. I source: /usr/local/ccp4-6.1.2/bin/ccp4.setup-sh before

[ccp4bb] Coot installation on MacBook Pro

2009-06-25 Thread Mohinder Pal
Hi! I have recently bought MacBook Pro and I have specific problem with installing coot on it. I already followed Scott lab's instructions but the problem still exists. All help and suggestions are most welcomed. Cheers! Mohinder