[ccp4bb] xtal programs free to non-academic users

please name some possible choices for data processing, structure determination, refinement and validation? Thank you Rongjin Guan

Re: [ccp4bb] statistics of A/B

how about Delta method? see examples here: http://www.math.umt.edu/patterson/549/Delta.pdf = = = = = = = = On 2011-06-07 08:47:04 You wrote = = = = = = = = Thank you for the reply. Hypothesis: A and B are independent and normal distribution. Could you please explain more? Thanks. --- On

[ccp4bb] reference on the van der Waals contact distance

curious how these numbers were developed. For example, S has a larger vdW radius than C, but the vdW contact cut-offs are the same as for C. Does any one know such an old reference or textbook for these numbers? Thanks Rongjin Guan

[ccp4bb] non-identical complexes in the asymmetric unit

have other explanations. Can anyone direct us to some references where this has been discussed before? Thank you very much Rongjin Guan

[ccp4bb] SAXS on a coiled coil protein

and linkers as commented by our collaborators who did the SAXS experiments. Could this be due to the shifting of the turn connecting the two helices? Has this kind of flexibility in the turn position in a coiled coil motif been observed in other coiled coil structures? Thank you Rongjin Guan

[ccp4bb] molprobity in coot: BL WARNING:: reduce didnt run ok, so stop here!

didnt run ok, so stop here! run_generic_script (probe, 0) My model was outputed from phenix refinement and I checked the format and can not see anything wrong. Can anyone give me some hints? Thanks Rongjin Guan

[ccp4bb] small molecule soaking screening

various conditions. Is this practical, if some of them have pretty similar binding affinities to the protein? All comments/suggestions are welcome. Thank you Rongjin Guan

[ccp4bb] cryo-protection for crystals grown in ethanol

that. Thanks, Rongjin Guan

[ccp4bb] detergent concentration used for membrane protein purification

before purification? any suggestions will be appreciated. Have a nice holiday! Rongjin Guan

[ccp4bb] phosphorylation of protein is troublesome or not for crystallization

Dear all, Below is a question my friend asked me, but I have never worked on phosphorylated proteins. Has anyone worked on crystallizing phosphorylated proteins and can you comment on it? Thanks Rongjin Guan -- I would