Dear all,
I am working with a cyclic protein and I am trying to make a figure with
ccp4mg. I would like to know how to say to ccp4mg that the N and C-terminus are
bound Although atoms are at a covalent bond distance, the chain is broken
by ccp4mg...
Many thanks in advance for your
,
Maria
Quoting Stuart McNicholas:
On 15/11/2012 12:26, SANCHEZ BARRENA, MARIA JOSE wrote:
Dear all,
I am working with a cyclic protein and I am trying to make a figure with
ccp4mg. I would like to know how to say to ccp4mg that the N and
C-terminus are bound Although atoms
Dear all,
I would like to share with you some problems I am experiencing with a protein,
in case someone has an idea about what it is going on:
I am working with a protein module that gets trapped in Ni-NTA or glutathione
beads. If the protein is purified without tag (ion exchange plus gel
Dear all,
I am trying to express a eukatiotic protein (E. coli codon optimized sequence)
with a GST tag at the N-terminus. I always get my overexpressed protein and a
contaminant around 60kDa. This contaminant is not washed out of the column when
washing glutathione beads with 1M NaCl-buffer.
Dear Gerard,
you are right, the abstract submission has been extended.
Best regards,
Maria
Quoting Gerard Bricogne:
Dear Harry,
Thank you for the reminder. Upon connecting to the URL given in
Martin's e-mail, however, one immediately gets a pop-up saying:
New
