Category: Scientific
Job ID: R-102000
Location: Burnaby, BC, CA
Additional Location: Canada - Mississauga
Posted Date: 7/24/2020
Summary
Amgen is a global biotechnology company that discovers and develops
breakthrough therapies to treat serious human illnesses. Our mission is to
serve patients
Category: Scientific
Job ID: R-102328
Location: Burnaby, BC, CA
Additional Location:
Posted Date: 7/29/2020
Title: Senior Scientist – Protein Technologies Team Lead
Reports to: Principal Scientist
Location: Burnaby
*JOB SUMMARY*
Amgen is a global biotechnology company that discovers and
?
Shane Caldwell
shane.caldwel...@gmail.com
rigid domains and a flexible
connecting region, as it uses local structural alignments so keeps hinged
movements from having a disproportionate impact on the alignment.
Shane Caldwell
McGill University
On Tue, Jul 7, 2015 at 9:03 PM, Keller, Jacob kell...@janelia.hhmi.org
wrote:
Is anyone aware
.
Shane Caldwell
McGill University
On Fri, Jul 3, 2015 at 2:56 AM, Kay Diederichs
kay.diederi...@uni-konstanz.de wrote:
On Thu, 2 Jul 2015 13:25:07 -0400, Edward A. Berry ber...@upstate.edu
wrote:
My take on this-
No one has been willing to specify a cutoff (and probably
Shouldn't the ability to distinguish enantiomers also depend upon the
degree of asymmetry of the particle itself? (or pseudosymmetry, I suppose)
With SAXS it should be easier to distinguish right-handed and left-handed
lock washers than it is to tell a right-handed from a left-handed wall
screw.
Shane Caldwell
McGill
On Tue, Jun 23, 2015 at 5:25 PM, sreetama das somon_...@yahoo.co.in wrote:
Dear All,
I have a transferase, which is showing broad specificity for both the
substrates (nucleotides) in our organism of interest, but is highly
specific in other organisms.
Are there any
It's probably much less likely than metal coordination and it's hard to
judge from only one angle, but phospho-histidine might be something else to
consider.
http://www.jbc.org/content/276/5/3247.full
Shane
On Mon, Jun 22, 2015 at 2:15 PM, Roger Rowlett rrowl...@colgate.edu wrote:
I
, especially if TLS is behaving weirdly, is to
check with the PARVATI server (http://skuld.bmsc.washington.edu/parvati/).
If your TLS group boundaries set off flags, you have some more work to do
on the refinement.
Shane Caldwell
McGill University
On Thu, Jun 18, 2015 at 4:30 PM, Christian Roth
Hi Smith,
You're possibly looking at a turn of 3_10 helix:
http://en.wikipedia.org/wiki/310_helix
Whether it's real or not will of course depend on your electron density.
Shane Caldwell
McGill University
On Fri, May 29, 2015 at 11:35 AM, Smith Liu smith_liu...@163.com wrote:
Dear All
, or ribonucleosides? Because that would
be the first place to look, if you haven't already.
Shane Caldwell
McGill University
On Thu, May 28, 2015 at 8:12 AM, Faisal Tarique faisaltari...@gmail.com
wrote:
Hello everyone
I am working on a 5'-nucleotidase (Mg as a cofactor) and have a decent
structure
is happening before or after purification.
Hope this helps,
Shane Caldwell
McGill University
On Tue, May 26, 2015 at 1:45 AM, Mohammad Khan mohdkhan0...@gmail.com
wrote:
Dear all,
I am working on a His-tag recombinant protein with two domains, which I
purify using affinity chromatography
Hi Vijay,
In addition to the suggestions you've already received, the pair_fit
command in PyMol is also an easy way to align based on specific atoms. You
could use the C-alphas of relevant active site residues, or atoms of the
ligand itself, for example. Just be mindful of the bias introduced
Of note, this discussion was recently in the pages of Nature:
http://www.nature.com/news/rule-rewrite-aims-to-clean-up-scientific-software-1.17323
Shane Caldwell
McGill University
On Tue, May 12, 2015 at 12:48 PM, James Stroud xtald...@gmail.com wrote:
I hereby call on the broadest community
this
doesn't mess up the dot product test. You'll end up with one number
in
each row having more than three decimal places.
Dale Tronrud
On 4/1/2015 2:52 PM, Shane Caldwell wrote:
Hi ccp4bb,
I'm trying to solve a problem I never quite figured out in the
past. I'd
good enough to pass the orthonormal test.
.. scratch that, it passes sometimes and still fails for other
structures/chains. So I'm still in search of a higher-precision export
Shane
On Wed, Apr 15, 2015 at 11:44 AM, Shane Caldwell shane.caldwel...@gmail.com
wrote:
Hi Bernhard, thanks
, Shane Caldwell shane.caldwel...@gmail.com
wrote:
good enough to pass the orthonormal test.
.. scratch that, it passes sometimes and still fails for other
structures/chains. So I'm still in search of a higher-precision export
Shane
On Wed, Apr 15, 2015 at 11:44 AM, Shane Caldwell
Hi Natalia,
This might be a good place to start:
http://www.sciencedirect.com/science/article/pii/S0968000404002348
The power of two: protein dimerization in biology.
Trends Biochem Sci. 2004 Nov;29(11):618-25
Marianayagam NJ1, Sunde M, Matthews JM.
Abstract: The self-association of proteins
with one number in
each row having more than three decimal places.
Dale Tronrud
On 4/1/2015 2:52 PM, Shane Caldwell wrote:
Hi ccp4bb,
I'm trying to solve a problem I never quite figured out in the past. I'd
like to use the *sortwater* utility to send my picked waters to various
users
do any more. I have quite a few structures with hundreds of waters each and
I'd like to get the waters organized, but doing it by hand will take a very
long time. Any help getting this program running would be greatly
appreciated!
Shane Caldwell
McGill University
Don't forget, multiplicity has its own negative connotations.
http://www.imdb.com/title/tt0117108/
Shane Caldwell
McGill University
On Sun, Jan 18, 2015 at 12:26 PM, Edward A. Berry ber...@upstate.edu
wrote:
Also RAID (REDUNDANT array of inexpensive disks). To me redundancy implies
Doubled once: http://imgur.com/Vy8oJfx
Doubled twice: http://imgur.com/q3gO0cI
Cheers,
Shane Caldwell
McGill University
On Fri, Jan 9, 2015 at 4:57 PM, Paul Emsley pems...@mrc-lmb.cam.ac.uk
wrote:
On 09/01/15 21:08, Shane Caldwell wrote:
Hi ccp4bb,
Apologies for a cross-post. I previously
!
Shane Caldwell
McGill University
multiplicity on e.g. a Cu source.
(Disclaimer: I'm no expert. Others here know how this works much better
than I do)
Shane Caldwell
McGill University
On Tue, Dec 9, 2014 at 8:35 AM, R. M. Garavito rmgarav...@gmail.com wrote:
Yamei,
This is not unusual, particularly for many proteins that bind nucleotide
not be as straightforward as MS
Cheers,
Shane Caldwell
McGill University
On Tue, Aug 19, 2014 at 2:56 AM, rohit kumar rohit...@gmail.com wrote:
Dear All,
i have solved a structure of 3.2 A. That is a PLP depended enzyme.
In their resting state, PLP-dependent enzymes are usually joined
of the subject I know of is Chapter 8 of
McPherson, although I don't think it addresses dimers specifically, just
nonspecific aggregates. Perhaps still a place to start?
Shane Caldwell
McGill University
On Wed, Aug 13, 2014 at 5:40 PM, Todd Jason Green tgr...@uab.edu wrote:
Hello All-
I am interested
see is really
protein and not simply crystals of adenosine. Note that crystals of
adenosine may still give you a signal under UV fluorescence, so best to
trust other tests for protein, or of course the one test that really
matters, diffraction.
Shane Caldwell
McGill University
On Fri, May 9, 2014
Hi SDY,
It would be unlikely unless you have other reasons to believe it (biology,
surrounding environment), but that blob looks a little like a possible
phospho-histidine. It might be worth a looking into?
That said, buffer or metals are probably the more likely explanation.
Shane Caldwell
the
stability of your electrode over time to get an idea.
Shane Caldwell
McGill University
On Thu, Jan 30, 2014 at 10:40 AM, Katherine Sippel
katherine.sip...@gmail.com wrote:
Alternatively you could make a stock solution of citric acid (say 1 M for
example) and stock solution of sodium citrate
!
Shane Caldwell
McGill University
QuikChange in a PreScission or TEV site in place of the thrombin site -
they tend to be much nicer proteases, and much more specific.
Hope this helps,
Shane Caldwell
McGill University
On Wed, Jan 22, 2014 at 5:59 AM, Pablo Power ppo...@ffyb.uba.ar wrote:
Dear Venkat,
I totally agree with Nicholas
Hi Omi,
Just to add to Preben's suggestion, something that jumps out from the
conditions you list - those are all fairly chaotropic anions. It might also
be worth looking into other anions from the same end of the Hofmeister
series (i.e. bromide), and possibly chaotropic cations as well.
Shane
if (3) is scientifically sound and/or will lead to
problems refining in refmac. What would be my best option?
Interested to hear thoughts on this, thanks!
Shane Caldwell
McGill University
in one of
the packages I already use, but if so, I can't seem to find it. Thanks in
advance for your help.
Cheers,
Shane Caldwell
McGill University
, but perhaps still
worth a look.
Cheers,
Shane Caldwell
McGill University
On Mon, Apr 22, 2013 at 8:20 PM, Pascal Egea pas...@msg.ucsf.edu wrote:
Thanks to All for the diligent answers to my query,
The protein is not thermophilic and has only one cysteine. We are working
in presence of freshly added
and QWERTY'd in?
/wild speculation
Shane Caldwell
McGill University
On Tue, Mar 19, 2013 at 9:46 AM, Edward A. Berry ber...@upstate.edu wrote:
Opher Gileadi wrote:
Hi Theresa,
To add to Anat's comments: Although the AUG codon for the first
methionine and all other methionines in a protein
Hi James,
The first place I'd look is oxygen conc, as you'll get different aeration
from different vessel shapes/sizes. You could play around with shaking
speed and/or flask geometry to try to get more or less aeration.
Shane Caldwell
McGill University
On Tue, Jan 15, 2013 at 9:48 AM, Murray
Hi Andrey,
Are you sure it's not PLP? To me that looks like you have confounding
aggregation, and PLP in two forms, perhaps only partly incorporated when
considering your protein absorbance. Check out the spectra in the paper:
Turning pyridoxal-5'-phosphate-dependent enzymes into thermostable
of attack.
Thanks for your time,
Shane Caldwell
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