[ccp4bb] Predefining origin for molecular replacement in polar space group

Dear all, Is there a way to specify (somewhat arbitrarily) a origin for any molecular replacement package (in a polar space group) without resorting to phased translation so that one could more easily compare results from different MR runs? Thanks, Shao-Yang -- Shao-Yang Ku EIPOD Post

Re: [ccp4bb] degradation of protein durring freez thaw

From your description, the protein concentration dropped from 10mg/mL to 1-2mg/mL after freeze-thaw. It's hard to imagine that your protein has been degraded. Degraded by what (protease)? At -80oC? Did you see a ladder of lower bands on the gel after the freeze-thaw? If you're interested

Re: [ccp4bb] Acta E

Quoting Bernhard Rupp b...@ruppweb.org: I suppose most have read the Acta D editorial already http://journals.iucr.org/d/issues/2010/01/00/me0408/me0408.pdf but it seems another creative way of structure generation has been discovered by some small molecule people:

Re: [ccp4bb] small molecule library

? Thanks alot already in advance for your help. Sincerely, Christopher -- Shao-Yang Ku EIPOD Post-doctoral fellow Schneider Group EMBL c/o DESY Notkestr. 85 22603 Hamburgphone: 0049-(0)40-89902-160 Germany email: s...@embl-hamburg.de

Re: [ccp4bb] Devices Suitable to Concentrate Protein Solutions in Liter Scale

Can you put a 6His-tag on your protein and add some Ni-NTA beads to your solution to capture (and concentrate) the properly folded molecules? Quoting Xuan Yang pattisy...@gmail.com: Dear All, I am working on protein refolding via dialysis in large volumn (typically 2~4 litters). It was

Re: [ccp4bb] soaking with Samarium chloride

Hi Amit, I wouldn't worry about acetate depleting the Sm3+. I have tried to make a derivative of a kinase by soaking ions of lanthanoid series but in the presence of phosphate buffer and ADP. In this case, lanthanoid ions are *known* to precipitate with phosphate (and phosphoryl moiety).