[ccp4bb] Postdoctoral position in macromolecular crystallography, HZI Braunschweig, Germany

of Proteins” headed by Prof. Wulf Blankenfeldt. The department focuses on unraveling molecular mechanisms of uncharacterized processes in the metabolism, regulation and virulence of pathogenic microorganisms. About the institute More than 800 people work at the HZI, a research institution

[ccp4bb] Off-topic: ITC - what to buy?

Dear all: we are looking into buying an isothermal titration calorimeter, but are only aware of two manufacturers: TA Instruments and MicroCal (GE Healthcare). Are there any more? Do you have any recommendations (brand, model etc.) for us? Thanks in advance, Wulf -- Prof. Dr. Wulf

[ccp4bb] SHELXL eating REFMAC5 waters - whom to trust?

Dear all, I am blessed/tormented with a 0.8 A data set for a small protein. I first refined it using COOT/REFMAC5 until I deemed it done, which meant manually adding quite a few water molecules that were weak and a little more distant to the protein. R is 10.2%, R-free is 10.8%. I realize

[ccp4bb] Making really good chemically competent cells

Dear all, please excuse the non-CCP4-related question, but maybe there is someone out there who can help us. We are trying to find a recipe for making REALLY good chemically competent E. coli for our local cloning facility. What we would like to have is more or less: - 109 cfu/ug DNA -