Dear CCP4BB Community,
I would like to comment you guy a review of the last five years of
high-throughput protein crystallization screening that would be a
magnificent help for all scientists that struggle with macromolecular
crystallization.
Please see attachment, as well as below:
https://ww
ndustry. Hopefully the cost of the robotic equipment
> will come down.
>
> -Daniel
>
>
> On Thu, Apr 26, 2018 at 11:43 AM, Yibin Lin wrote:
>
>> Dear CCP4BB Community,
>>
>> I would like to comment you guy a review of the last five years of
>> hi
Hi forks,
I got some membrane protein crystals in DDM. However, after one month,
these crystals change to Irregular shape, and have not birefringent in
polarized Light. Do anybody know why? Does the effect of DDM? Contain
too much detergent?
Thanks,
Zhang Peng
Hi fork,
I am working with a membrane protein. I got some crystals from peg400.
All of these crystals don't diffract. I tried to use a needle to break
crystal. They didn't break into small one, look like gel, or oil,
something like that. Do anybody has deal about these crystals.
Detergent crystal?
Hi List,
I want to preprare sel-met labeling protein. Could someone can tell me
if it is necessary to add DTT or BME to medium, which contains
sel-met, when growing cells?
Thanks in advance,
Yibin Lin
Use robot. You only need 0.1ul*96=9.6ul of protein solution.
On Thu, Mar 3, 2011 at 7:59 PM, m zhang wrote:
> Dear all,
>
> I am trying to optimize my crystal with additives. Since the yield of my
> protein purification is very limited, I am wondering what is the most
> efficient way to set u
ou have a very
> small amount present, say as a contaminant with citrate or EDTA, it will
> crystallize.
>
> On Feb 21, 2011, at 1:22 PM, Yibin Lin wrote:
>
> > Dear all,
> >
> > I got a lot of salt crystals in reservior solution (well solution), which
> conta
Dear all,
I got a lot of salt crystals in reservior solution (well solution), which
contains 0.1 M phosphate/citrate ph 4.2, PEG200 47%, EDTA-2Na 0-22mM.
Reservior solution appears crystals from 12mM EDTA. Could someone help me to
explain why?
Thank you very much!
Yibin
Actually, it is very difficult to say something just based on your simple
information. You should give more informations about your work, for example,
temperature, cuture, etc.
Best,
Lin
On Fri, Jan 21, 2011 at 8:12 PM, m zhang wrote:
> Dear All,
>
> Recently I am trying to express a glycoprote
Dear all,
I try to express selenomethinine-labeled protein in E.coli methinoine
auxotrophic strain B834 in M9 with 17 amino acid and 50 mg/ml se-met for 18
hours at 37 degree. I don't why the colour of medium vary from colorless to
yellow. Could anybody tell me it is normal or not?
Thanks a lot!
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