[ccp4bb] Relion MotionCorr error

Hi community, I've been trying to use Relion 3.0 beta2's own Motion Correction to process ~4000 movies collected by K3 camera. However, it stopped in the middle after 40 hours run with errors shown below. Does anyone has experience with it? Thanks in advance for your advice! Yu Qiu rawTIFF

Re: [ccp4bb] SO4 or PO4

Hi Shijun, I had a similar issue a while ago that a acetylated lysine binding site was occupied by acetate from buffer. In this case, I would suggest trying crystallize in a conditions without SO4, e.g. using NH4Cl instead, if it is reproducible. Best, Yu From: CCP4 bulletin board

[ccp4bb] protein quasicrystals?

Hi, I have been trying to crystallize a protein complex and keep getting sphere shape crystals. The diffraction is around 3 angstrom, but looks like multiple lattices. I am wondering if it could be a quasi crystal? Is there anyone has such experience? Thanks, Yu

[ccp4bb] Senior Principal Scientist Position at Sanofi US

Senior Principal Scientist, Protein Engineering, Biologics Research Sanofi Framingham, MA, US (Formerly Sanofi Genzyme) Apply here: