From: conan_...@hotmail.com
To: ccp4bb@jiscmail.ac.uk
CC: enz...@rice.edu
Subject: rosie server, wrong plots? (off topic)
Date: Tue, 14 Apr 2015 22:07:21 +
Dear All,
Any one knows why the Rosie Server for protein docking (docking2) gave two
plots that seem not consistent with each
Hi Faisal,
CC-half standard is valuable in evaluating the cut-off of highest resolution.
Sometimes even if I/sigI is close to 1 and completeness is not as high, if
CC-half is still significant, it may be worth incorporate the extra high-res
shell data and extend the resolution. Again, if only
Hi Shiv,
Michael has made good points. Also I found similar case, i.e, different loop
and active site catalytic residue conformations with slightly different PEG
2000 mme concentration. In my case the catalytic residue moves away from active
site and the loop more disordered in lower PEG
Min,
In some of my Xtal conditions, I only have 1.5 to 2M AmSO4 without KCl, the
salt comes out of solution only after maybe several minutes which is enough to
harvest the crystal. Maybe you could just direct freeze to try the diffraction
since it is already at high salt concentration or maybe
Dear All, Hi. I was asked in a manuscript revision to discuss about the
possible effects of Fourier transformation ripples on the crystallographic
results. Specifically, the reviewers question whether ripples may affect on the
electron density around heavy metal center which has a
