A trick I like is just to freeze the reservoir solution or would-be
cryo-solution without a crystal present. If the frozen solution stays clear and
does not show ice rings on e.g. a home source, it is worth trying. Otherwise,
the solution needs optimization.
Herman
-Ursprüngliche
Herman,
The trick you suggest is not as valid as you may think. The ice rings can
originate from the crystal itself.
If you crystallize in a high concentration PEG precipitant you will avoid
ice rings,
but if you transfer or soak your crystals in the same solution the high
molecular weight
@JISCMAIL.AC.UK] Im Auftrag von Enrico
Stura
Gesendet: Dienstag, 27. August 2013 11:58
An: CCP4BB@JISCMAIL.AC.UK
Betreff: Re: [ccp4bb] AW: [ccp4bb] cryoprotection
Herman,
The trick you suggest is not as valid as you may think. The ice rings can
originate from the crystal itself.
If you crystallize
