eanor Dodson [eleanor.dod...@york.ac.uk]
Sent: Wednesday, November 29, 2017 1:07 PM
To: Denis Rousseau
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] AW: Differences in a homodimer protein
I would submit the coordinates to PISA to analyse the buried surface area for
each momnomer and see if there are
29, 2017 10:32 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] AW: Differences in a homodimer protein
>
> I have seen this in MnSOD. We had a tetramer in the ASU and each
> active site had different ligands in our peroxide soak. I assumed the
> crystal lattice can influe
: Differences in a homodimer protein
I have seen this in MnSOD. We had a tetramer in the ASU and each
active site had different ligands in our peroxide soak. I assumed the
crystal lattice can influence these things or it is inappropriate
metal incorporation. I would highly recommend doing ICP-MS on a
I have seen this in MnSOD. We had a tetramer in the ASU and each
active site had different ligands in our peroxide soak. I assumed the
crystal lattice can influence these things or it is inappropriate
metal incorporation. I would highly recommend doing ICP-MS on a
dissolved crystal and on your pu
Dear Denis,
I would first superimpose both monomers to see if you can find a reason why one
subunit has a bound water and the other not, which would in general be flanking
side chains in (slightly) different positions. Next I would look for some
global differences between the subunits that coul
