Dear users, Pardon me for the non-crystallography related question, Can anyone provide some papers regarding the assay ofenzymes from hypertherphilic /thermophilic organisms? ie,assays at high temperatures..Thanking youWith RegardsM. Kavyashree --
This message has been scanned for viruses and
You can do it without having to do an assay at high temperature, because the
substrate stability would also change at higher temperature. What you could
do is the following:
1. Use a PCR machine to heat the enzyme, and then cool it down.
2. Keep one fraction at 4C / RT separately.
3. Do the
