Good points have been brought up; here's one more to consider from my
experience. If you are going to run SEC prior to crystallization, I would
highly recommend running a native gel of the peak you collect. Especially
if you don't know the stoichiometry or if the stoichiometry is complex. I
crystal
in or protein-nucleic acid complex and their crystallizability. I
would like to know, if you find any.
Chitta
- Original Message -
From: "Roger Pickman"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Friday, December 7, 2012 10:11:15 AM
Subject: [ccp4bb] Binding constan
Dear Roger,
I disagree with Ganesh. Knowing the stoichiometry is not necessary.
Stoichiometry
may need adjusting to reflect the relative solubility of the interacting
partners
under the various crystallization conditions.
See also:
Stura, E.A., Graille, M., Taussig, M.J., Sutton, B.J. Gore,
Dear Roger,
In my humble opinion, the qualitative knowledge that the complex
actually forms (established through pull down assays, gel filtration
etc) is probably far more important than the Kd values in solution. In
any case, the crystallization is done at very high concentrations, far
abov
Dear all - is there a rule of thumb for favourable values of Kd, kon and
koff of protein-protein or protein-dna complexes for protein
crystallisation? Are these measurements useful in crystallisation, or
should one just put it down a gel filtration column, hope for a complex and
not worry? If an
