2,3-BD users,
What sort of concentrations are you using?
Dave
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On Mar 22, 2010 11:45 PM, Steve Tuske tu...@cabm.rutgers.edu wrote:
Hi Ulrike,
2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems to decrease mosaicity. I was able
Hello Dave,
I have good experience with concentrations between 10% and 15%
2,3-butanediol. My typical starting concentration is 12.5%.
Good luck.
Eike
Am 23.03.10 08:27 schrieb David Briggs unter drdavidcbri...@gmail.com:
2,3-BD users,
What sort of concentrations are you using?
I would not rely on recipes. When I pick a cryo protectant, I always prepare a
set of solutions and take an image from a large loop dipped into solution.
Then I pick the one with the least concentration that does not show ice rings
anymore.
The behaviour can change with a crystal in the loop, but
20 percent (v/v).
Steve
2,3-BD users,
What sort of concentrations are you using?
Dave
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Delivered via an Android.
On Mar 22, 2010 11:45 PM, Steve Tuske tu...@cabm.rutgers.edu wrote:
Hi Ulrike,
2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems
Hi everybody,
has anyone experience with Butandiol and its different isoforms as
cryoprotectant ?
Thanks,
Ulrike
Hello Ulrike,
in our lab 2,3-Butanediol has had a good reputation.
Tim
On Mon, Mar 22, 2010 at 01:32:33PM +, Ulrike Demmer wrote:
Hi everybody,
has anyone experience with Butandiol and its different isoforms as
cryoprotectant ?
Thanks,
Ulrike
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Tim Gruene
Institut fuer
Hi Ulrike,
2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems to decrease mosaicity. I was able to switch from a
three-step soaking protocol with glycerol and glucose to a quick (30
second to one minute) soak with 2,3-BD.
It's a winner.
Steve
I
Hello
